Skip to main content
Figure 4 | Proteome Science

Figure 4

From: Effect of glutathione reductase knockdown in response to UVB-induced oxidative stress in human lung adenocarcinoma

Figure 4

2D-DIGE analysis of UVB-dependent differentially expressed proteins in CL1-0 and CL1-0ΔGR cells. UVB-irradiated CL1-0 and CL1-0∆GR cells were lysed and arranged for a triplicate 2D-DIGE experiment. Protein samples (150 μg each) were labeled with Cy-dyes and separated using 24 cm, pH 3–10 non-linear IPG strips. 2D-DIGE images of the protein samples from pooled cell lysates and UVB-irradiated CL1-0 and CL1-0∆GR cells at 45 mJ/cm2 were shown as well as overlaid pseudo-colored image processed with ImageQuant Tool (GE Healthcare). Green color spots represented proteins of CL1-0 cells and CL1-0 cells irradiated with UVB. Red color spots represented proteins of ∆GR cells and ∆GR cells irradiated with UVB. The merge was shown as yellow color (A). DeCyder software was further performed to detect and calculated differentially expressed proteins. The differentially expressed identified protein features are annotated with spot numbers (B).

Back to article page

Proteome Science

ISSN: 1477-5956