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Figure 2 | Proteome Science

Figure 2

From: The rat striatum responds to nigro-striatal degeneration via the increased expression of proteins associated with growth and regeneration of neuronal circuitry

Figure 2

Tyrosine hydroxylase (TH) immunoreactivity decreases by 3 days post-lesioning and is undetectable after 7 and 14 days post-lesioning. Tyrosine hydroxylase (TH) immunoreactivity in coronal sections through the striatum of (A) unlesioned), (B) 3 day, (C) 7 day, and (D) 14 day post-lesioned animals, highlights the ongoing loss of dopaminergic input to the striatum at time points used for proteomic analysis. Though there is a noticeable staining of numerous TH + process in the striatum of the unlesioned brain (A), only few swollen, varicose TH + process remain in the striatum at 3 days post 6-OHDA lesioning (arrow in B). As expected, there was no detectable staining of TH in the striatum after 7 days post lesioning (C), and this remained unchanged at 14 days (D) post 6-OHDA injection to the medial forebrain bundle. Scale bars in A-D = 10 μm. Striatal protein extracts were subjected to SDS-PAGE and transferred to nitrocellulose by electroblotting blotting (E). The blot was probed with an antibody against tyrosine hydroxylase – TH, with beta III tubulin as an internal loading control. PL = post 6-OHDA lesioning.

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ISSN: 1477-5956