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Figure 5 | Proteome Science

Figure 5

From: The rat striatum responds to nigro-striatal degeneration via the increased expression of proteins associated with growth and regeneration of neuronal circuitry

Figure 5

Western blotting verifies the expression levels of several proteins that were identified by iTRAQ analysis. Striatal protein extracts were subjected to SDS-PAGE and transferred to nitrocellulose either by electroblotting blotting (B), or by diffusion blotting to produce two identical blots from one gel (A, C, and D). Blots were probed with antibodies against (A) glial fibrillary acidic protein - GFAP, (B) neurofilament heavy polypeptide - NFh, (C) growth associated protein 43 - GAP43, or (D) dopamine- or cAMP- regulated neuronal phosphoprotein - DARPP-32, with beta III tubulin as an internal loading control in each case. PL = post 6-OHDA lesioning.

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ISSN: 1477-5956