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Table 2 Differentially expressed membrane and cytosolic protein fractions were identified with linear ion trap nano-LC-ESI-MS/MS

From: Proteomic analysis of Staphylococcus aureus biofilm cells grown under physiologically relevant fluid shear stress conditions

Spot ID

Fold changed

Protein name

XC score

Cov (%)

pI/Mr

Acc no

Funtional category

 

Membrane fraction: higher abundance in 1000S-1

20

-3.1

Malate dehydrogenase

170

54

4.9/44

gi:15924692

Carbohydrate metabolism

33

-2.5

Brached -chain alpha-keto acid dehydrogenase

260

42

4.8/46

gi:15924085

Carbohydrate metabolism

42

-2.2

50S ribosomal protein

80

58

4.6/17

gi:15923529

Amino acid metabolism

 

Membrane fraction: lower abundance in 1000S-1

2

5.8

Glyceraldehyde 3-phosphate dehydrogenase

106

30

5.9/37

gi:15924677

Carbohydrate metabolism

3

4.2

Glyceraldehyde 3-phosphate dehydrogenase

48

21

5.9/37

gi:15924677

Carbohydrate metabolism

4

4.2

Alanine dehydrogenase

258

66

5.5/40

gi:21283381

Carbohydrate metabolism

7

3.6

Malate:quinone oxidoreductase

443

62

6.1/56

gi:15925597

Carbohydrate metabolism

8

3.6

Alcohol dehydrogenase

510

76

5.2/36

gi:21282297

Carbohydrate metabolism

14

3.3

Ornithine--oxo-acid transaminase

340

81

5.1/43

gi:15923947

Amino acid metabolism

15

3.2

Ribosomal subunit interface protein

200

68

5.0/22

gi:15923742

Amino acid metabolism

17

3.1

Nucleoside diphosphate kinase

90

61

4.9/17

gi:15924459

Nucleotide Metabolism

21

2.9

Malate:quinone oxidoreductase

236

44

6.1/56

gi:15925597

Carbohydrate metabolism

25

2.8

Succinyl-CoA synthease

166

49

5.4/32

gi:15924236

Carbohydrate metabolism

27

2.7

Alanine dehydrogenase

148

47

5.0/40

gi:15924429

Carbohydrate metabolism

34

2.5

Superoxide dismutase

118

62

5.2/23

gi:15923123

Stress protein

37

2.4

F0F1 ATP synthase

80

43

5.7/32

gi:15925094

Carbohydrate metabolism

 

Cytosolic fraction: lower abundance in 1000S-1

1

0.71

Glyceraldehyde 3-phosphate dehydrogenase

48

21

5.9/37

gi:15924677

Carbohydrate metabolism

7

0.60

Ornithine--oxo-acid transaminase

340

81

5.1/43

gi:15923947

Amino acid metabolism

12

0.59

Alcohol dehydrogenase

516

76

5.2/36

gi:21282297

Carbohydrate metabolism

14

0.78

Succinyl-CoA synthetase

106

30

5.4/32

gi:15924236

Carbohydrate metabolism

19

0.77

Formate acetyltransferase

148

22

5.2/84

gi:15923216

Carbohydrate metabolism

20

0.63

Malate dehydrogenase

170

54

4.9/44

gi:15924692

Carbohydrate metabolism

26

0.55

Alcohol dehydrogenase

510

76

5.2/36

gi:21282297

Carbohydrate metabolism

33

0.67

50S ribosomal protein

80

36

10.2/24

gi:15925240

Amino acid metabolism

  1. Fold change was calculated using proteins in 50 s-1 compared to those in 1000 s-1. Protein functional categories were annotated from KEGG database.