Figure 1

Effects of forced expression of S100A10 on cell proliferation and chemosensitivity. (A) Untransfected COLO-320 cells (UT), cells transfected with non-target vector (control), cells stably transfected with S100A10 (S100A10), and HT29 cells used as a positive control were lysed. Cell lysates (10 μg protein) were subjected to western blot analysis using anti-S100A10 (1:5000), anti-annexin A2 (1:2500), and anti-GAPDH antibodies (1:4000). Molecular weight standards (MW) are in the left lane. (B) The growth curves of each cell line. (C) Each cell was treated with various concentration of L-OHP or 5-FU. Cell viability after exposure to L-OHP or 5-FU (upper panel), and IC₅₀ values for L-OHP or 5-FU (lower panel) were determined. Data are the mean ± SD (n = 4). P values are by comparison with the results for UT (**P < 0.01) and control (††, P < 0.01) by on-way ANOVA with Tukey’s post hoc test.