Figure 2
From: Forced expression of S100A10 reduces sensitivity to oxaliplatin in colorectal cancer cells
Effects of S100A10 or/and annexin A2 gene silencing on cell proliferation and chemosensitivity. HT29 cells were treated with transfection reagent alone (mock) or transfected with nontargeting control siRNA (control, final concentration 10 nM or 20 nM), S100A10 siRNA (S100A10 siRNA, final concentration 10 nM), annexin A2 siRNA (ANXA2 siRNA, final concentration 10 nM), or both S100A10 siRNA and annexin A2 siRNA (S100A10 siRNA + ANXA2 siRNA, final concentration 20 nM). (A) The expression levels of S100A10 and annexin A2 were examined by using real-time qRT-PCR (lower panel) and western blot analyses (upper panel). (B) Cell proliferation was determined after transfection for the indicated times (left panel, single knockdown; right panel, double knockdown). Data are the mean ± SD (n = 3). P values are by comparison with the results for the mock (*, P < 0.05), control (††, P < 0.01), or S100A10 siRNA (§, P < 0.05; §§, P < 0.01) by one-way ANOVA with Tukey’s post hoc test. (C) Cell viability after exposure to L-OHP was determined. Cells were treated with various concentrations of L-OHP after single knockdown with each siRNA (left panel) or after double knockdown of S100A10 and annexin A2 (right panel). Data are the mean ± SD (n = 9).