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Table 1 Fifty-seven proteins with an individual percentage of equal to or larger than 0.1% constitute 98% of the total identified proteome

From: The Lottia gigantea shell matrix proteome: re-analysis including MaxQuant iBAQ quantitation and phosphoproteome analysis

Protein Accession-no. % of total identified proteome Phospho-rylation
Aspartate-, glycine-, lysine- and serine-rich protein/B3A0P1/peroxidase-like protein 1; domain: haem_peroxidase (~aa40-675); 20% G, 12% S; pI 4.96; GO: extracellular; DS: most of aa680-1870 Lotgi1|162078 DGLSP_LOTGI2 16.71 ++
Proline-rich protein 1/B3A0Q1; 11% A, 13% P; pI:9.72; GO: extracellular; DS: C-terminal 15aa Lotgi1|2354971 PRP1_LOTGI2 12.28 (+)
Glycine- and methionine-rich protein/B3A0R1; 12% A, 20% G, 10% L, 18% M, pI:11.24; GO: extracellular; DS: aa125-225 Lotgi1|2391741 GMP_LOTGI2 9.14  
Glycine- and Serine rich protein-1/B3A0P6; 10% A, 20% G, 13% S; pI 9.0; GO: extracellular; DS: ~aa67-84 (18aa) Lotgi1|239214 GSP1_LOTGI2 6.82 (+)
Peroxidase-like protein 2/B3A0P3; domains: haem_peroxi-dase (~aa666-1124); 13% G, 11% S; pI 8.52; GO: extracellular; DS: ~aa1-620, aa1197-1492 Lotgi1|2328171 PLSP2_LOTGI2 6.80 ++
Glycine-rich protein/B3A0R2; 10% A, 16% G, 12% M, 10% L; pI 9.87; GO: extracellular; DS: aa127-145 (19aa) Lotgi1|2391701 GRP_LOTGI2 5.91  
Uncharacterized shell protein 5/B3A0Q0; 13% A, 11% R, 11% L; pI 10.32; GO: extracellular; DS: short stretches especially in C-terminal half Lotgi1|2388311 USP5_LOTGI2 5.11  
Coiled-coil domain-containing protein 1/B3A0Q3; domain: coil; 31% D; pI 3.55; GO: extracellular; DS: short stretches all over aa27-394 Lotgi1|2334201 CCD1_LOTGI2 3.49 ++
Similar to blue mussel shell protein (BMSP)/ similar to collagen α4 (VI); domains: VWA; 11% I; pI 8.33; GO: extracellular; DS: none Lotgi1|1406601 Lotgi1|1731392 2.81  
Uncharacterized shell protein 13/B3A0R3; 10% G; pI 8.32; GO: extracellular; DS: ~aa180-291 Lotgi1|2348851 USP13_LOTGI2 2.13  
Uncharacterized shell protein 16/B3A0R5; pI 9.63; GO: extracellular; DS: none Lotgi1|2310461 USP16_LOTGI2 2.01  
Proline-rich protein 2/B3A0R8; 16% P; pI 9.98; GO: extracellular; DS: short stretches especially in aa161-186 Lotgi1|2305101 PRP2_LOTGI2 1.67  
Glycine-, glutamate-and proline-rich protein/B3A0P5; domain: Lysozyme_like (~aa240-415); 12% Gly; pI 5.08; GO: extracellular; DS: aa73-137, aa201-218 Lotgi1|2313111 GEPRP_LOTGI2 1.45 +
Methionine-rich protein/B3A0R7; 10% N, 11% P; pI 9.62; GO: extracellular; DS: ~aa50-400 Lotgi1|1732001 MRP_LOTGI2 1.43  
Uncharacterized shell protein 26/B3A0P4/BMSP-like; 18% G, 12% S, 10% T; pI 9.11; GO: extracellular; DS: small segments scattered over entire sequence Lotgi1|2385261 USP26_LOTGI2 1.42 +
Uncharacterized shell protein 8/B3A0Q4; 11% P, 10% Y; pI 9.71; GO: extracellular; DS: short regions interspersed throughout the sequence Lotgi1|2282681 USP8_LOTGI2 1.22 +
Uncharacterized protein; 10% Q (C-term), 11% P; pI 9.67; GO: none; DS: : small segments scattered over entire sequence Lotgi1|1581131 1.19  
Uncharacterized/similar to superoxide dismutase; domain: SOD; 12% P, 10% Q; pI 9.30; GO: intracellular/extracellular; DS: ~aa20-450; SOD:~aa480-635 Lotgi1|166131 Lotgi1|1016111 1.09 +
SCP domain-containing protein 2/B3A0P8; domain: CAP (~aa145-310); pI 9.56; GO: extracellular; DS: ~aa16-155 Lotgi1|2332001 SCP2_LOTGI2 0.97  
Similar to nacrein-like protein/putative carbonic anhydrase 1/B3A0P2; domain: α-carbonic anhydrase; pI 6.44; GO: extracellular; DS: none Lotgi1|2380821 CAH1_LOTGI2 0.96  
Putative carbonic anhydrase 2; aa190-632 100% identity to CAH2/B3A0Q6; domain: α-CA (~aa85-411); 11% R, 13% D, 13% G; pI 5.87; GO: extracellular; DS: aa415-633 Lotgi1|2391881 CAH2_LOTGI2 0.88  
Uncharacterized protein; 10% A, 12% L; pI 9.77; GO: extracellular; DS: few to none Lotgi1|2310091 0.87  
Uncharacterized protein; domain: CBM_14 (chitin-binding)/peritrophin A (~aa18-87); pI 6.65; GO: extracellular; DS: none Lotgi1|1731381,2 0.87  
Uncharacterized protein; domain: IGFBP_Nterm; 11% C, 10% S; pI 9.03; GO: extracellular; DS: none Lotgi1|1740651 0.81  
Uncharacterized shell protein 4/B3A0P9; 10% S, 12% Y; pI 8.89; Go: extracellular; DS: possibly short C-tern segment Lotgi1|2361831 USP4_LOTGI2 0.77  
Glycine and tyrosine-rich protein/B3A0Q2; 14% G, 13% T; pI 5.43; GO: extracellular; DS: most of the sequence Lotgi1|2356211 GTRP_LOTGI2 0.71  
aa151-448 96% identity to coiled-coil domain-containing protein 2/B3A0Q7; 10% D, 20% G (GM/GGG-rich C-terminus (~aa430-630); pI 3.77; GO: extracellular; DS: most of aa290-410 Lotgi1|234936 CCD2_LOTGI2 0.67  
Uncharacterized protein; domains: antistasin, WAP; 16%C,11% P, pI 5.62; GO: extracellular; DS: none Lotgi1|2391251 Lotgi1|226725 0.66  
Uncharacterized protein/glycosidase 2; domain: DUF187; similar to GEPRP_LOTIA (37% identity); pI 4.76; GO: extracellular; DS: ~aa78-130 Lotgi1|1749201,2 0.64 +
Uncharacterized protein/similar to ER aminopeptidase; domain: peptidase_M1, ERAP1_LIKE_C; pI 8.94; GO: ER/Golgi/ext. plasma membrane; DS: none Lotgi1|1407861 Lotgi1|225855 0.61  
SCP domain-containing protein 1/B3A0P7; domain: CAP (aa143-305); 11% S; pI 9.21; GO: extracellular; DS: ~aa20-110 Lotgi1|2331991 SCP1_LOTGI2 0.53  
Uncharacterized Gly-rich protein; 12% N, 22% G; pI 9.54/9.30; GO: extracellular; DS: ~aa40-200 (275200) Lotgi1|2394471 Lotgi1|175200 0.47  
Similar to chorionic proteinase inhibitor/perlwapin-like; domains: WAP (5x); aa1-125 99.6% identity to B3A0S1; 11% C, 10% P; pI 7.84; GO: extracellular; DS: none Lotgi1|239121 Lotgi1|201802 PWAPL_LOTGI2 0.39  
Uncharacterized protein; pI 9.49; GO: none; DS: none Lotgi1|2343871 0.38  
Proline-rich protein 3/B3A0S4; 10% N, 11% G, 13% P; pI 9.56; GO: extracellular; DS: few short segments Lotgi1|2379961 Lotgi1|172116 PRP3_LOTGI2 0.34  
EGF-like domain-containing protein 1 (aa170-682 of entry)/B3A0R6; domains: EGF (aa241-277), zona _pellucida (ZP; aa284-534); pI 5.80; GO: extracellular; DS: ~aa525-620 Lotgi1|2355481 ELDP1_LOTIA2 0.27  
Peroxidase-3/B3A0Q8; domain: haem_peroxidase (aa531-1077); 13% N; pI 7.5; GO: extracellular; DS: 26-381 Lotgi1|2328181 Lotgi1|99809 PLSP3_LOTGI2 0.26  
Uncharacterized protein/LUSP_10; 16% A, 17% D; pI 3.82; GO: extracellular; DS: most of the sequence Lotgi1|1636371,2 0.25  
Uncharacterized protein; Pro/Ala- and His-rich motifs in C-term; pI 8.78; GO: extracellular; DS: short segments scattered over entire sequence Lotgi1|2333971 Lotgi1|163339 0.24  
Similar to peptidyl-prolyl cis/trans isomerase/B3A0R0; domain: cyclophilin_type_PPI; 13% G; pI 4.75; GO: extracellular; DS: none Lotgi1|2229791 Lotgi1|169679 PPI_LOTGI2 0.24  
Uncharacterized; domains: VWC/pacifastin; 13% C, 12% D, 11% S; pI 3.87; GO: extracellular; DS: none Lotgi1|2308541 Lotgi1|99757 0.23  
Uncharacterized Gln-rich protein; 26% Q, 13% L, 12% T; pI 4.02; GO: extracellular; DS: ~aa40-320 Lotgi1|1593311 0.22  
Uncharacterized Pro-rich protein; 15% P; pI 9.50: GO: extracellular; DS: aa32-416 Lotgi1|1740031 0.22  
Uncharacterized protein/LUSP-18; 15% P, 15% T; pI 5.73; GO: extracellular; DS: ~aa18-557 Lotgi1|2356101,2 0.20  
EGF-like domain_containing protein 2/B3A0S3; domains: EGF (aa73-109), ZP (aa116-370); pI 4.9; GO: extracellular; DS: few (aa364-386,403-425) Lotgi1|167423 ELDP2_LOTGI2 0.19  
Uncharacterized protein/Similar to PIF; 41% identity to PIF_PINFU aa427-526; domain: ConA_like_lectin; pI 8.91; GO: extracellular; DS: none Lotgi1|2375101 Lotgi1|171086 0.16  
Uncharacterized protein/LUSP-14; domain: chitin_binding_3; pI 8.77; GO: extracellular; DS: aa225-251 Lotgi1|2267261 Lotgi1|2391292 0.16 +
Uncharacterized protein; 28% identical to PIF_PINFU: domains: VWA, chitin-binding, ConA_like_lectin; pI 5.15; Go: extracellular; DS: none Lotgi1|2282641 0.15  
Uncharacterized Gln-rich protein; domain: Sushi/SCR/CCP (aa158-212); 19% Q, 11% P; pI 9.19; GO: extracellular; DS: most of the sequence Lotgi1|2348841 Lotgi1|166202 0.14  
Uncharacterized protein; aa1-138 100% identity to ASRP/B3A0S2; 10% A, 10% N, 19% D, 11% V; pI 3.73 acid C-term half); GO: extracellular; DS: aa43-232 Lotgi1|2383581 ASRP_LOTGI2 0.14 +
Uncharacterized protein; 13% S; pI 4.43; GO: extracellular; DS: aa47-338 Lotgi1|1710841 0.11 +
Perlustrin-like/B3A0Q9; 43% identity to PLS_HALLA; domain: IGFBP_N; 11% C, 11% E; pI 4.05; GO: extracellular; DS: none Lotgi1|2389701 PLSLP_LOTGI2 0.11  
Uncharacterized protein; 10% Q, 10% P, 11% S; pI 9.79; GO: extracellular; DS:~aa90-928 Lotgi1|1583161 0.10  
Uncharacterized protein; domain: SOUL_haem_binding; 13% L; pI 6.96; GO: extracellular; DS: none Lotgi1|2050301 Lotgi1|237594 0.10  
Uncharacterized protein; 11% E, pI 4.32; GO: none (transmembrane?); DS: aa426-669 and smaller segments Lotgi1|1540201 0.10 ++
Uncharacterized shell protein 22/B3A0S0; 21% Q, 18% P; pI 8.43; GO: extracellular; DS: most of the sequence Lotgi1|2366901 USP22_LOTGI2 0.10  
Uncharacterized protein/LUSP-20; domains: chitin_binding CBM_14/peritrophin A (aa384-504); 13% T; pI 6.79; GO: extracellular; DS: most of ~ aa60-380 Lotgi1|2395741,2 0.10  
  1. +, less than three peptides phosphorylated; ++, three or more phosphopeptides; (+), not confirmed with phosphopeptide-enriched samples. DS, predicted disordered structure. 1, previously identified by Mann et al., 2012 [17]; 2, previously identified by Marie et al., 2013 [18].