Protein expression and transcription profiles of three strains of Aeromonas salmonicida ssp. salmonicida under normal and iron-limited culture conditions

Table 3 List of the primers used in this study

Primer Name	Sequence	Gene targeted	Amplicon size
FdAS-16SRNART	ATATTGCACAATGGGGGAAA	16S RNA	148 bp
RvAS-16SRNART	GTTAGCCGGTGCTTCTTCTG	16S RNA	148 bp
FdAS-AHPCRT	CCTACCACAATGGCAAGTT	Alkyl Hydroperoxide Reductase C	180 bp
RvAS-AHPCRT	TCGGTGGAGACGGAGTAGA	Alkyl Hydroperoxide Reductase C	180 bp
FdAS-AtpF RT	CGGTCAGACACTTGCTTTCA	atpF gene product	149 bp
RvAS-AtpFRT	TGGCCAAATCCAGATCTTTC	atpF gene product	149 bp
FdAs-EnoRT	GATACGACCTTCGCCTTTCA	Enolase	112 bp
RvAs-EnoRT	TGCTGCTGGCTATGTACTGG	Enolase	112 bp
FdAS-H-NS-RT	CGTTTGAACAGCTGCAAGAA	hns Transcriptional regulator	127 bp
RvAS-H-NS-sRT	ATTTCGGTGAACTCGCTCAG	hns Transcriptional regulator	127 bp
FsAS-VapART	TGTTCCGGTTCCTGTTGTT	Virulence array protein (VapA)	177 bp
RvAS-VapART	CACGAATGTGGATGAGGTTG	Virulence array protein (VapA)	177 bp

ISSN: 1477-5956