Figure 1
From: Protein phosphorylation differs significantly among ontogenetic phases in Malus seedlings
2-DE images of differentially expressed phosphorylated proteins in different ontogenetic phases of apple seedling 02-18-081. The seedling 02-18-081 was derived from a cross hybrid ‘Jonathan’ × ‘Golden Delicious’, the proteins were extracted from leaf sample. Juvenile, adult vegetative and reproductive phases are designated by J, V and R, respectively. Proteins were profiled in the first dimension by isoelectrofocusing using linear IPG stripes (pH 3–10, 24 cm) and on SDS-polyacrylamide gels in the second dimension. Phosphorylated protein groups are indicated by open squares. Panel a corresponds to global proteins stained with Coomassie Brilliant Blue R-350, panel b represents total phosphorylated proteins stained with Pro-Q Diamond, and panel c shows dephosphorylated proteins pre-treated with calf intestinal phosphatase and stained with Coomassie Brilliant Blue R-350. Approximate molecular masses and isoelectric points are indicated on the right edge and top margin, respectively.