Figure 5

Quantitative Real-time RT-PCR analysis of the mRNA expression levels of TCTP in various plants by PCD induction. The morphology of Arabidopsis was induced by dark for 0, 24, 36, 48, 60, 72 h (A). qRT-PCR analysis of TCTP mRNA expression levels in dark-induced Arabidopsis for 0, 24, 36, 48, 60, 72 h (B). The morphology of rice was induced by dark for 0, 24, 36, 48, 60, 72 h (C). qRT-PCR analysis of TCTP mRNA expression levels in dark-induced rice for 0, 24, 36, 48, 60, 72 h (D). DNA laddering in rice protoplasts after heat treatment was detected by agarose electrophoresis. Protoplasts were treated at 48°C for 15 min and allowed to recover at 28°C for 0, 2, 4, 6 h. Lane M: Marker; Lane 1: untreated (ck); Lane 2: recovered for 0 h; Lane 3: recovered for 2 h; Lane 4: recovered for 4 h; Lane 5: recovered for 6 h (E); qRT-PCR analysis of TCTP mRNA expression levels in heat shock rice protoplasts for ck, 0, 2, 4, 6 h (F). qRT-PCR analysis for quantitative analysis of hsr203J (G) and TCTP (H) mRNA expression levels after TMV-infected tobacco for 0, 1, 3, 5, 7, 10 h. Data are displayed as mean values ± SD from three independent experiments. The same letter indicated no significant difference, and different letters indicated significant differences, as determined by Fisher’s protected LSD test (p < 0.05).