Figure 6

Quantitative Real-time RT-PCR analysis of the mRNA expression levels of SAMS2, DAMDC and polyamines, ethylene contents in maize leaves under flooding treatment for 0, 1, 2, 3, 4 d. qRT-PCR assays for quantitative analysis of SAMS2 (A) and SAMDC (B) mRNA expression levels under flooding treatment. HPLC assays of polyamine content in maize leaves under flooding treatment; putrescine (PUT), spermidine (SPD) and spermine (SPM) (C). Gas chromatograph (GS) assays of ethylene content in maize leaves under flooding treatment; Ethylene contents (D). Data are displayed as mean values ± SD from three independent experiments. The same letter indicated no significant difference, and different letters indicated significant differences, as determined by Fisher’s protected LSD test (p < 0.05).