Table 2 Specific primers used for RT-PCR analysis
Gene | Forward primer | Reverse primer |
|---|---|---|
ZmCyclophilin (Zea may) | 5’-atcgtgatggagctgtacgccaa-3’ | 5’-tggcacatgaacacggggat-3’ |
ZmTCTP (Zea may) | 5’-cactaatccgacattcctcta-3’ | 5’-aacaagcaatccaatcttgg-3’ |
zm50s RPL (Zea may) | 5’-ggttgtgagatgttgtat-3’ | 5’-aagaaggatgtatatgttgt-3’ |
ZmPH (Zea may) | 5’-gctacttacgcagagaat-3’ | 5’-caccagagttccacataa-3’ |
zmDREPP4 (Zea may) | 5’-gaaggaagaggaggataagc-3’ | 5’-ttaccagcagcaagcaag-3’ |
zmFpPKIWI502 (Zea may) | 5’-actactgaacattacatc-3 | 5’- taatcatctcatctcctt-3 |
zmHSP70 (Zea may) | 5’-aggaggtggactaagcggat-3 | 5’-cttaaaacgcgtgccacgat-3 |
ZmADK (Zea may) | 5’-agaaggtcctcccgtatgct-3 | 5’-tgcctgaagccaaaggaagt-3 |
ZmMDH (Zea may) | 5’-cagcggaatgcatttgcca-3 | 5’-tgcatcatagtcaaattcgtgtgg-3 |
zmOEE1 (Zea may) | 5’-gaccgccgtcatggatctt-3 | 5’-aaaagcgacagcccgaat-3 |
ZmPGK (Zea may) | 5’-gcgctagcctggaattgttg-3 | 5’-tcggtagcagacctccgtaa-3 |
ZmPEPCK (Zea may) | 5’-aaacgatggtgtgtgtgcgt-3 | 5’-acaagaccagagaccagacg-3 |
ZmFbp (Zea may) | 5’-ctccaacgaggtgttctcca-3 | 5’-gaacacgacgatgtagttgcc-3 |
zm3-Bhsd (Zea may) | 5’-gacgctgtcggagaaccatt-3 | 5’-cagggggtgcaaggattagg-3 |
zmHrBP1 (Zea may) | 5’-gctcagggtgtttgtcat-3 | 5’-aggaagaaggatggcaatc-3 |
zmChitinase (Zea may) | 5’-gtcaccaacatcatcaac-3 | 5’-caagcaagtcacagtatc-3 |
18S rRNA (Oryza) | 5’- cctatcaactttcgatggtaggata-3’ | 5’-cgttaagggatttagattgtactcatt-3’ |
TCTP (Oryza) | 5’-ttcctttacttctctcat-3’ | 5’-tcatctcaacatccataa-3’ |
eEF1α (Nicotiana) | 5’-taatgtggttcttagttc-3’ | 5’-agttccgaattaagtatc-3’ |
hsr203j (Nicotiana) | 5’-cagagttcatcaacaagcatta -3’ | 5’-acaatcaagacggtacatca-3’ |
TCTP (Nicotiana) | 5’-tcaaggagcaaccaagta-3’ | 5’-caccattccagtatcatcag-3’ |
Actin (Arabidopsis thaliana) | 5’-gccatccaagctgttctctc-3’ | 5’-gctcgtagtcaacagcaacaa-3’ |
TCTP (Arabidopsis thaliana) | 5’-ctgtgttggaagattctca -3’ | 5’-agattcgggagtttaatttaga -3’ |
HRT (Zea may) | 5’-aagaacctcagaggcaaagc-3’ | 5’-acattctcccagaaggctgc-3’ |
CLA (Zea may) | 5’-gctgactccgactttgacga-3’ | 5’-ctgctttcctgtcgtccctt-3’ |
SAMS2 (Zea may) | 5’-ccctttcggtgttcgtgga-3’ | 5’-acagcacagcactgcaacat-3’ |
zm-Plmbp (Zea may) | 5’-ctttgctgggctgatttccg-3’ | 5’-tcttgccttcgtctcgcaat-3’ |
IAA/AUIX (Zea may) | 5’-ttacagtccagagcaaga-3’ | 5’-tacagaccataaggcagaa-3’ |
ACS6 (Zea may) | 5’-agctgtggaagaaggtggtcttcgaggt-3’ | 5’-agtacgtgaccgtggtttctatga-3’ |
ACO15 (Zea may) | 5’-ctcgtcttcgatcaattcccaagt-3’ | 5’-tacattatcattatttctccggctgt-3’ |
SAMDC2 (Zea may) | 5’-gaaaggcacttggtgcagag-3’ | 5’-ccgtcaaagcagtggaaaaca-3’ |