Table 4 Resume of primary and secondary antibodies used for western blot validation along with dilutions and incubation times
From: The plasma proteomic signature as a strategic tool for early diagnosis of acute coronary syndrome
Protein | Primary antibody | Dilution | Incubation | Secondary antibody | Dilution | Incubation |
|---|---|---|---|---|---|---|
Gamma fibrinogen | Abcam | 1:20000 | 1 hour | - | - | - |
Hemopexin (NSTEACS) | Abcam | 1:2000 | 1 hour | Rabbit True blood eBioscience | 1:1000 | 45 min |
Apolipoprotein E | Abcam | 1:500 | 1 hour | IgG antigoat donkey HRP Abcam | 1:2000 | 45 min |
Antithrombin III (NSTEACS) | Abcam | 1:2000 | 1 hour | Mouse True blood eBioscience | 1:1000 | 45 min |
Alpha-1 antichymotrypsin | Abcam | 1:2000 | 1 hour | Mouse True blood eBioscience | 1:1000 | 45 min |
Apolipoprotein AI | Polyclonal | 1:50000 | 1 hour | Rabbit True blood eBioscience | 1:10000 | 45 min |
Coagulation Factor XII | Abcam | 1:1000 | 1 hour | Rabbit True blood eBioscience | 1:10000 | 45 min |
Antithrombin III (STEACS) | Abcam | 1:2000 | 1 hour | Mouse True blood eBioscience | 1:1000 | 45 min |
Hemopexin (STEACS) | Abcam | 1:200 | 1 hour | Rabbit True blood eBioscience | 1:10000 | 45 min |
Kallistatin | Abcam | 1:2000 | 1 hour | Rabbit True blood eBioscience | 1:10000 | 45 min |