Figure 5
From: Glucose induces rapid changes in the secretome of Saccharomyces cerevisiae
Experimental Designs for iTRAQ1 and iTRAQ2, and the distribution of proteins identified in iTRAQ1 vs iTRAQ2. (A) Schematic drawing of the iTRAQ1 and iTRAQ2 experiments examining changes in t30/t0 ratios. Wild-type cells were grown in YPKG or transferred to YPD for 30 minutes. Extracellular proteins were extracted from t = 0 and t = 30 cells and digested with trypsin. In iTRAQ1, peptides from replicates of t = 0 cells were labelled with 113 and 114, whereas peptides from replicates of t = 30 cells were labelled with 115 and 116. We used 115/113 (t30a/t0a) and 116/114 (t30b/t0b) to identify proteins that showed altered t30/t0 ratios. In iTRAQ2, peptides from t = 0 cells were labelled with113, whereas peptides from t = 30 cells were labelled with 114. We then used 114/113 (t30c/t0c) to identify proteins that showed changes in t30/t0 ratios. (B), BioVenn analysis[100] was used to compare proteins identified in iTRAQ1 and iTRAQ2. In this current study, 241 proteins overlapped in both iTRAQ1 and iTRAQ2, whereas 23 were uniquely identified in iTRAQ1 and 83 proteins were uniquely identified in iTRAQ2.