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Table 1 Identification of fourteen hypothetical proteins in mouse hippocampus and their putative functions

From: Altered expression of hypothetical proteins in hippocampus of transgenic mice overexpressing human Cu/Zn-superoxide dismutase 1

     

Theoretical/Observed

Accession-number

Protein name

Putative function b / localization

BLAST Alignment (identity to, %)

Matches (Score) c

p I

MW (kDa)

Metabolism-related proteins

O54983

Mu-crystallin homolog

Ornithine cyclodeaminase / cytoplasm

Q9QYU4 (97%)

14 (124)

5.44 / 5.55

33.52 / 34.20

Q99KI0

Similar to mitochondrial aconitase

Aconitase / cytoplasm or mitochondria

Q9ER34 (97%)

18 (122)

8.08 /7.80–8.00

85. 46 / 85.40

Q9CWS0

2410006N07Rik protein

Amidinotransferase / cytoplasm

O08557 (98%)

23 (199)

5.64 / 5.65

31.38 / 35.20

Q9CXN1

3110052N05Rik protein

Sugar phosphatase / cytoplasm

Q9H008 (38%)

11 (133)

5.70 / 5.73

28.73 / 28.50

Q9D051

2610103L06Rik protein

Pyruvate dehydrogenase E1, beta subunit / mitochondria

P11177 (93%)

12 (66)

6.41 / 5.58

38.94 / 32.20

Q9D0K2a

2610008O03Rik protein

Succinyl-CoA: 3-ketoacid transferase / mitochondria

P55809 (87%)

9 (68)

8.73 / 7.82

55.99 / 52.10

Q9D2G2

4930529O08Rik protein

Dihydrolipoamide succinyltransferase / mitochondria

P36957 (76%)

15 (116)

9.10 / 5.74

48.99 / 49.20

Signaling-related proteins

Q9D358

4632432E04Rik protein

Protein tyrosine phosphatase / neclear

Q9QWF5 (99%)

6 (59)

6.13 / 5.72

17.92 / 15.55

Q9D8Y0

Swiprosin 1

Predicted Ca2+-binding protein, EF-hand protein superfamily / cytoplasm

NH

16 (155)

5.01 / 5.10

26.79 / 30.85

Transcription-related proteins

Q99KP6

Hypothetical protein

mRNA splicing factor / cytoplasm

 

9 (75)

6.14 / 6.25

55.24 / 53.00

Q99LX0

Similar to DJ-1 protein

Putative transcriptional regulator / cytoplasm

O88767 (95%)

18 (156)

6.32 / 6.08

20.02 / 20.85

Others

AAA59434d

Germline-encoded VH3 immunoglobulin binding- locus

Immunoglobulin binding / cytoplasm

P23083 (96%)

5 (73)

9.43 / 5.32

11.20 / 32.50

Q61786

ORF 1

Transposase / nuclear

NH

9 (63)

10.07 / 7.00

32.48 / 49.10

Q9NVA2

Hypothetical protein FLJ10849

Cell division-associated protein / cytoplasm

Q14141 (80%)

18 (173)

6.36 / 6.40

49.40 / 49.30

  1. Mouse hippocampus proteins were separated by 2-DE and identified by MALDI-TOF, following in-gel digestion with trypsin. The spots representing the identified proteins are indicated in Fig 1 and are designated with their accession numbers of SWISS-PROT or dNCBI database. The number of matching peptides (Matches), probability of assignment (Score), theoretical molecular weight (MW), and pI values are given. a Identified in rat brain (Unpublished data, Shin et al., 2003) bPrediction of function of hypothetical proteins was performed by sequence-motif approach (Pfam, http://www.sanger.ac.uk/Pfam/) and NCBI database (BLAST search, http://www.ncbi.nlm.nih.gov/BLAST) and putative localization resulted from PSORT II http://www.psort.org using of k-nearest neighbour (k-NN) algorithm for assessing the probability of localising at each candidate sites. c Score is – 10*Log (P), where P is the probability that the observed match is a random event (MASCOT, http://www.matrixscience.com). NH, there was no comparable homologs with known function
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