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Figure 2 | Proteome Science

Figure 2

From: Validation of a prefractionation method followed by two-dimensional electrophoresis – Applied to cerebrospinal fluid proteins from frontotemporal dementia patients

Figure 2

Reproducibility study of direct and prefractionated 2-DE of CSF on SYPRO Ruby stained 2-D gels. a) Represents a standard 2-D gel image of prefractionated (using LP-IEF) CSF fractions with pH 4.5–6.0, separated on a pH 4–7 IPG-strip. Numbers represents the CVs of encircled protein spots from four individually prefractionated, identical CSF samples separated by 2-DE. The mean CV of the 20 marked spots is 14.6% ± 7.9% (mean ± SD). b) Represents a standard 2-D gel image of directly analysed (after acetone precipitation) CSF proteins, separated on a pH 4–7 IPG-strip. Numbers represents the CVs of encircled protein spots from four replicate gels. The mean CV of the 20 marked spots is 15.4% ± 8.3% (mean ± SD). Molecular weights (MW) are in kDa.

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