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Table 5 Array 1: Averaged LLQs/ULQs in pg/mL obtained from 15 point standard titrations

From: Development and standardization of multiplexed antibody microarrays for use in quantitative proteomics

Feature

B

W

A

W+A

LLQ (pg/mL)

ULQ (pg/mL)

ANG

1

6

93

99

7

1262

BLC

7

93

0

93

62

4614

EGF

32

65

3

68

347

1184

ENA-78

8

81

12

92

161

9225

Eot

19

81

0

81

186

4454

Eot2

2

83

15

98

23

2313

FGF-7

28

72

0

72

218

19367

FGF-9

67

33

0

33

463

21049

Fas

16

84

0

84

290

39248

GDNF

66

34

0

34

48

7715

GM-CSF

67

33

0

33

67

3626

IL-13

29

71

0

71

29

4458

IL-15

73

27

0

27

4682

52224

IL-1ra

45

54

0

55

71

8960

IL-2sRa

3

94

3

97

20

3975

IL-3

58

42

0

42

852

19428

IL-4

81

19

0

19

43

3548

IL-5

67

33

0

33

13

2438

IL-6

59

34

7

41

14

2291

IL-7

73

27

0

27

32

2396

IL-8

21

65

13

79

6

916

MCP-2

13

84

4

87

42

2144

MCP-3

45

54

1

55

58

3205

MIP-1a

51

46

3

49

464

10315

MPIF-1

9

91

0

91

293

7410

OSM

73

27

0

27

78

8511

PlGF

46

54

0

54

61

3080

  1. Data shown is from titrations run in 8 client projects (1000 clinical samples surveyed) covering 8 diverse disease areas. The percentage of samples falling below (B), within (W) or above (A) the linear range of detection are presented. The analyte was considered to be detectable if the W+A percentage was above 50%.