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Table 5 Array 1: Averaged LLQs/ULQs in pg/mL obtained from 15 point standard titrations

From: Development and standardization of multiplexed antibody microarrays for use in quantitative proteomics

Feature B W A W+A LLQ (pg/mL) ULQ (pg/mL)
ANG 1 6 93 99 7 1262
BLC 7 93 0 93 62 4614
EGF 32 65 3 68 347 1184
ENA-78 8 81 12 92 161 9225
Eot 19 81 0 81 186 4454
Eot2 2 83 15 98 23 2313
FGF-7 28 72 0 72 218 19367
FGF-9 67 33 0 33 463 21049
Fas 16 84 0 84 290 39248
GDNF 66 34 0 34 48 7715
GM-CSF 67 33 0 33 67 3626
IL-13 29 71 0 71 29 4458
IL-15 73 27 0 27 4682 52224
IL-1ra 45 54 0 55 71 8960
IL-2sRa 3 94 3 97 20 3975
IL-3 58 42 0 42 852 19428
IL-4 81 19 0 19 43 3548
IL-5 67 33 0 33 13 2438
IL-6 59 34 7 41 14 2291
IL-7 73 27 0 27 32 2396
IL-8 21 65 13 79 6 916
MCP-2 13 84 4 87 42 2144
MCP-3 45 54 1 55 58 3205
MIP-1a 51 46 3 49 464 10315
MPIF-1 9 91 0 91 293 7410
OSM 73 27 0 27 78 8511
PlGF 46 54 0 54 61 3080
  1. Data shown is from titrations run in 8 client projects (1000 clinical samples surveyed) covering 8 diverse disease areas. The percentage of samples falling below (B), within (W) or above (A) the linear range of detection are presented. The analyte was considered to be detectable if the W+A percentage was above 50%.