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Table 6 Array 2: Averaged LLQs/ULQs in pg/mL obtained from 15 point standard titrations

From: Development and standardization of multiplexed antibody microarrays for use in quantitative proteomics

Feature B W A W+A LLQ (pg/mL) ULQ (pg/mL)
AR 73 27 0 27 48 5599
BDNF 8 87 5 92 26 3956
Flt3Lig 2 98 0 98 17 9835
GCP-2 26 74 0 74 132 13153
HCC4 8 46 46 92 112 7907
I-309 30 70 0 70 20 5142
IL-17 95 5 0 5 330 7921
IL-1a 69 31 0 31 10 2943
IL-1b 46 54 0 54 4 2932
IL-2 89 7 4 11 31 3671
M-CSF 55 45 0 45 86 6761
MCP-1 17 69 14 83 82 2065
MIG 8 87 5 92 13 5030
MIP-1b 29 56 16 71 16 2399
MIP-1d 10 72 18 90 192 6969
NT-3 75 25 0 25 160 22314
NT-4 60 40 0 40 128 19170
PARC 4 25 70 96 12 1870
Rantes 0 13 87 100 5 1302
SCF 29 71 0 71 69 20306
TARC 15 85 0 85 21 3134
TNF-R1 2 97 1 98 108 16285
TNF-a 71 29 0 29 56 6901
TNF-b 88 12 1 12 221 7617
VEGF 86 14 0 14 702 79564
sgp130 0 63 37 100 334 38573
  1. Data shown is from titrations run in 8 client projects (1000 clinical samples surveyed) covering 8 diverse disease areas. The percentage of samples falling below (B), within (W) or above (A) the linear range of detection are presented. The analyte was considered to be detectable if the W+A percentage was above 50%.