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Table 7 Array 3: Averaged LLQs/ULQs in pg/mL obtained from 15 point standard titrations

From: Development and standardization of multiplexed antibody microarrays for use in quantitative proteomics

Feature B W A W+A LLQ (pg/mL) ULQ (pg/mL)
BTC 84 16 0 16 980 25361
DR6 7 93 0 93 1200 86864
FGF1 60 40 0 40 162 53854
FasL 79 21 0 21 4300 82436
Fractalkine 78 22 0 22 662 12170
GROb 6 91 3 94 66 2505
HCC1 3 28 70 97 164 3610
HGF 70 30 0 30 1039 80079
HVEM 6 94 0 94 636 105560
ICAM-3 0 100 0 100 477 126635
IGFBP2 0 40 60 100 1523 46325
IL2Rg 71 29 0 29 429 41325
IL5Ra 63 37 0 37 1945 57281
IL-9 36 64 0 64 4506 156041
L-Selectin 0 47 53 100 161 28007
Leptin 5 69 26 95 1322 58408
MCP4 21 74 6 79 93 2508
MIP3b 18 82 0 82 40 9211
MMP7 0 99 1 100 70 21138
MMP9 0 51 49 100 3043 163481
PECAM1 6 93 1 94 1033 52935
RANK 17 83 0 83 223 87693
SCF R 3 97 0 97 445 38465
ST2 51 49 0 49 558 66234
TIMP1 0 77 23 100 2010 90647
TRAIL R4 87 13 0 13 4945 122116
VEGF R2 17 83 0 83 1254 138750
  1. Data shown is from titrations run in 8 client projects (1000 clinical samples surveyed) covering 8 diverse disease areas. The percentage of samples falling below (B), within (W) or above (A) the linear range of detection are presented. The analyte was considered to be detectable if the W+A percentage was above 50%.