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Table 7 Array 3: Averaged LLQs/ULQs in pg/mL obtained from 15 point standard titrations

From: Development and standardization of multiplexed antibody microarrays for use in quantitative proteomics

Feature

B

W

A

W+A

LLQ (pg/mL)

ULQ (pg/mL)

BTC

84

16

0

16

980

25361

DR6

7

93

0

93

1200

86864

FGF1

60

40

0

40

162

53854

FasL

79

21

0

21

4300

82436

Fractalkine

78

22

0

22

662

12170

GROb

6

91

3

94

66

2505

HCC1

3

28

70

97

164

3610

HGF

70

30

0

30

1039

80079

HVEM

6

94

0

94

636

105560

ICAM-3

0

100

0

100

477

126635

IGFBP2

0

40

60

100

1523

46325

IL2Rg

71

29

0

29

429

41325

IL5Ra

63

37

0

37

1945

57281

IL-9

36

64

0

64

4506

156041

L-Selectin

0

47

53

100

161

28007

Leptin

5

69

26

95

1322

58408

MCP4

21

74

6

79

93

2508

MIP3b

18

82

0

82

40

9211

MMP7

0

99

1

100

70

21138

MMP9

0

51

49

100

3043

163481

PECAM1

6

93

1

94

1033

52935

RANK

17

83

0

83

223

87693

SCF R

3

97

0

97

445

38465

ST2

51

49

0

49

558

66234

TIMP1

0

77

23

100

2010

90647

TRAIL R4

87

13

0

13

4945

122116

VEGF R2

17

83

0

83

1254

138750

  1. Data shown is from titrations run in 8 client projects (1000 clinical samples surveyed) covering 8 diverse disease areas. The percentage of samples falling below (B), within (W) or above (A) the linear range of detection are presented. The analyte was considered to be detectable if the W+A percentage was above 50%.