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Table 1 Amino acid sequence of neuroglobin peptides identified from human CSF using CapLC nanoESI Q-TOF tandem mass spectrometry.

From: Human neuroglobin protein in cerebrospinal fluid

#a

Amino Acids

M/z b

Zc

Molecular Weight

Δd

Amino Acid Sequences

Timee (min)

    

Calc.

Exp.

   

1

1–10

423.98

3

1268.65

1268.91

0.25

(-)MERPEPELIR(Q)

37.02

1

15–30

580.73

3

1738.95

1739.18

0.23

(R)AVSRSPLEHGTVLFAR(L)

42.94

1

19–30

442.99

3

1325.71

1325.95

0.25

(R)SPLEHGTVLFAR(L)

42.68

1

131–146

580.04

3

1736.87

1737.10

0.24

(R)AAWSQLYGAVVQAMSR(G)

68.23

1

131–151

761.42

3

2281.06

2281.23

0.17

(R)AAWSQLYGAVVQAMSRGWDGE(-)

74.49

2

131–146

580.02

3

1736.87

1737.03

0.16

(R)AAWSQLYGAVVQAMSR(G)

68.24

2

131–151

761.37

3

2281.06

2281.09

0.03

(R)AAWSQLYGAVVQAMSRGWDGE(-)

74.54

3

1–10

423.97 f

3

1268.65

1268.88

0.23

(-)MERPEPELIR(Q)

37.31

3

15–30

435.82 f

4

1738.95

1739.27

0.32

(R)AVSRSPLEHGTVLFAR(L)

43.27

3

19–30

442.99

3

1325.71

1325.96

0.25

(R)SPLEHGTVLFAR(L)

43.01

  1. a. Subject Number
  2. b. Mass / charge ratio
  3. c. Charge
  4. d. Difference (error) between the experimental (Exp.) and calculated (Calc.) molecular weights
  5. e. Elution time expressed in minutes.
  6. f. Peptides in Subject 3 identified only with ProteinLynx Global Server using the SwissProt database.