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Figure 2 | Proteome Science

Figure 2

From: Enhanced detergent extraction for analysis of membrane proteomes by two-dimensional gel electrophoresis

Figure 2

2-DE of RBC ghost membranes extracted with A) 4% CHAPS, B) 3% CHAPS : 1% LPC, C) 3% CHAPS : 1% MEGA 10, D) 3% CHAPS : 0.5% LPC : 0.5% MEGA 10. Extractions were carried out in buffer with 8 M urea, 2 M thiourea, protease inhibitor cocktail, and the indicated detergent for 1 hour on ice. Gels are representative of three independent experiments. Roman numerals indicate areas of improvement including i, the multiple transmembrane spanning protein band III.

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