Western blot of in vitro expressed proteins generated on the workcell. Variants are identified by row (letter) and column (number) on the azo-CMC plate (Figures 6 and 7) and by method used to pick colonies, either manual (MP) or automated (AP). Colors of boxes above lanes correspond to colors of boxes around cleared zones for those variants on plates in Figures 6 and 7. Wild-type CelF and CelF variants of similar length are at 47.7 kD. E5MP at 52.6 kD had a mutated stop codon resulting in a variant with 54 more amino acids than wild-type. Band for GUS is seen at 60 kD.