Skip to main content

Advertisement

Figure 5 | Proteome Science

Figure 5

From: Competition between bound and free peptides in an ELISA-based procedure that assays peptides derived from protein digests

Figure 5

Theoretical determination of the optimal conditions for the Peptidomatrix. (A) Binding of the antibody (at different dilutions) to wells containing increasing amounts of peptide,0.1(),1(), 10(), 100(). (B) Titration of bound peptide (different amounts) with increasing concentrations of antibody, 0.1(), 1(), 10(), 100(). (C) Competition between attached and free peptide using 4 concentrations of attached peptide,0.1(),1(), 10(), 100(), first antibody 'dilution' was 10. (D) Competition between attached and free peptide using 4 concentrations of antibody 0.1(),1(), 10(), 100(), 'attached peptide' was 10. All these curves are simulations and were drawn using equation A2 (for panel A and B) and A5 (for panel C and D), with kinetic parameters of Ka = 30, Kp = 10, cap = 50 and Kf = 1, see the appendix for details. All the data were analyzed using the Michaelis-Menten equation (normal and descending hyperbola). The derived values of Kd and Amax appear in table 5.

Back to article page