Optimising the Use of TRIzol-extracted Proteins in Surface Enhanced Laser Desorption/ Ionization (SELDI) Analysis

Table 1 Comparison of the number of peaks detected in direct and TRIzol extractions using UREA-CHAPS reagent and CM10 array.

		Number of peaks detected by CM10
Laser Power	M/Z Range (x1000)	Direct extraction	TRIzol extraction
		Urea	Urea	Triton	ACN	SDS
Low	2 to 10	14	15	14	12	2
High	10 to 200	60	60	45	33	12
Total	2 to 200	74	75	59	45	14

ISSN: 1477-5956