Figure 1

Representative SELDI-TOF MS spectra of pooled CSF sample obtained from all tested conditions using SPA as matrix. CSF was processed on CM10, Q10, H50 and IMAC30 ProteinChip arrays prepared under the following conditions: CM10 a: Native – 100 mM ammonium acetate, pH 4.0 b: Native – 100 mM ammonium acetate/0.1% Triton X-100, pH 4.0 c: Denatured – 100 mM ammonium acetate, pH 4.0 d: Denatured – 100 mM ammonium acetate/0.1% Triton X-100, pH 4.0 e: Denatured/reduced – 100 mM ammonium acetate, pH 4.0 f: Denatured/reduced – 100 mM ammonium acetate/0.1% Triton X-100, pH 4.0 Q10 a: Native – 100 mM Tris-HCl, pH 9.0 b: Native – 100 mM Tris-HCl/0.1% Triton X-100, pH 9.0 c: Denatured – 100 mM Tris-HCl, pH 9.0 d: Denatured – 100 mM Tris-HCl/0.1% Triton X-100, pH 9.0 e: Denatured/reduced – 100 mM Tris-HCl, pH 9.0 f: Denatured/reduced – 100 mM Tris-HCl/0.1% Triton X-100, pH 9.0 H50 a: Native – 10% AcN/0.1% TFA b: Native – PBS c: Denatured – 10% AcN/0.1% TFA d: Denatured – PBS e: Denatured/reduced – 10% AcN/0.1% TFA f: Denatured/reduced – PBS IMAC30 a: Native – 100 mM Cu sulfate,100 mM Na phosphate/0.5 M NaCl, pH 7.0 b: Native – 100 mM Ni sulfate, 100 mM Na phosphate/0.5 M NaCl, pH 7.0 c: Denatured – 100 mM Cu sulfate, 100 mM Na phosphate/0.5 M NaCl, pH 7.0 d: Denatured – 100 mM Ni sulfate, 100 mM Na phosphate/0.5 M NaCl, pH 7.0 e: Denatured/reduced – 100 mM Cu sulfate, 100 mM Na phosphate/0.5 M NaCl, pH 7.0 f: Denatured/reduced – 100 mM Ni sulfate, 100 mM Na phosphate/0.5 M NaCl, pH 7.0