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Table 2 Comparison of peak count between all tested conditions using CHCA as matrix.

From: Optimization and evaluation of surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry for protein profiling of cerebrospinal fluid

   Native 9 M urea, 2% CHAPS 9 M urea, 2% CHAPS, 10 mM DTT
   Peak count Average Peak count Average Peak count Average
CM10 w/o Triton X-100 66 68 98 90 76 79
   70   86   85  
   69   86   78  
  Triton X-100 75 76 77 77 68 67
   76   84   68  
   75   72   66  
Q10 w/o Triton X-100 86 86 91 87 82 75
   86   89   74  
   86   82   70  
  Triton X-100 77 75 73 81 75 71
   73   89   70  
   74   81   70  
H50 10% AcN, 01% TFA 71 71 85 81 73 73
   72   80   73  
   71   80   73  
  PBS 60 62 63 63 59 58
   64   63   61  
   62   65   55  
IMAC30 Copper 93 89 99 104 77 82
   85   109   81  
   88   106   89  
  Nickel 90 89 87 87 74 81
   88   85   85  
   89   91   84  
  1. Peaks with a signal-to-noise of 3 or greater were assigned between the m/z range of 2.5–20 kDa. 5 μL of pooled CSF prepared under native, denaturing, and denaturing/reducing buffer was applied in triplicate to each condition tested on CM10, Q10, H50, and IMAC30 arrays.
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