Figure 5
From: Proteomic analysis of tyrosine phosphorylation during human liver transplantation
Determination of Nck-1 interactants by GST pull-down. A. Schematic representation of the approach used for tyrosine mass spectrometry identification of GST Nck-1 binding proteins. GST Nck-1 matrix: GST Nck-1 fusion protein coupled to sepharose beads. B. Representative SDS-PAGE experiment after GST Nck-1 pull-down of 0 (I0) and 60 min ischemia (I60) and 0 (R0) and 60 min reperfusion (R60) protein extract. Bands corresponding to GST Nck-1 fusion protein and cleavage products (Nck-1 and GST) are marked by arrows. N = 1 on 2 independent pools of 3 liver biopsy protein extracts. C. Venn diagram representation of the proteins identified after mass spectrometry analysis of GST Nck-1 binding proteins.