Table 2 Anti-PY binding proteins – 60 min reperfusion
From: Proteomic analysis of tyrosine phosphorylation during human liver transplantation
Protein name | Accession number¶ | Mascot score | Sequence Coverage | Peptide number | Subellular Localization | Biological Function | Reported PY sites* | Predicted PY sites+ |
|---|---|---|---|---|---|---|---|---|
Actin, cytoplasmic 2 | P63261 | 664 | 42% | 19 | cytosol | cytoskeleton | none | 108,164,365,476,521 |
Carbamoyl-phosphate synthetase 1 | Q5R206 | 538 | 9% | 11 | mitochondrion | detoxification | none | 443,634,768,852,872,957, 959,1032,1305,1450 |
60S ribosomal protein L7 | P18124 | 495 | 45% | 10 | cytosol-nucleus | protein metabolism | none | 51,155,159,182 |
Serum albumin precursor | P02768 | 393 | 17% | 8 | secreted | transport | none | 108,164,365,476,521 |
Alcohol dehydrogenase [NADP+] | P14550 | 387 | 21% | 8 | cytosol | detoxification | none | 49,139,240,324 |
Unknown (protein for MGC:22633) | BC022319 1 | 375 | 21% | 8 | unknown | unknown | none | 14,341 |
Dihydrolipoamide branched chain transacylase | P11182 | 349 | 17% | 8 | mitochondrion | energy metabolism | none | 36,85,130 |
Myosin heavy chain | P13533 | 316 | 6% | 7 | cytosol | cytoskeleton | none | 162,284,311,387,411,502, 1377,1462,1490,1854 |
Protein disulfide isomerase-related protein | Q14554 | 218 | 10% | 3 | ER | protein metabolism | none | 113,222,242,245,357,364, 488 |
60S ribosomal protein L6 | Q02878 | 217 | 16% | 4 | cytosol-nucleus | protein metabolism | none | 68,72,239 |
60S ribosomal protein L13a | P40429 | 210 | 27% | 6 | cytosol-nucleus | protein metabolism | none | 53,191 |
60S ribosomal protein L18 | Q07020 | 175 | 24% | 4 | cytosol-nucleus | protein metabolism | none | 23,165 |
60S ribosomal protein L5 | P46777 | 143 | 12% | 3 | cytosol-nucleus | protein metabolism | none | 29,30,43,48,144,182,218, 239, 252 |
Enoyl-CoA hydratase | P30084 | 107 | 3% | 2 | mitochondrion | energy metabolism | none | 35 |
Very-long-chain acyl-CoA synthetase | O14975 | 102 | 4% | 2 | ER | detoxification | none | 81, 117, 261, 413, 460, 553, 610 |
Ku antigen, 70 kD | NP001460 2 | 102 | 4% | 2 | nucleus | nucleobase metabolism | none | 8, 30, 103, 361, 534, 559 |
Alpha-s1-casein | P47710 | 102 | 10% | 2 | secreted | transport | none | none |
60S ribosomal protein L8 | P62917 | 99 | 9% | 3 | cytosol-nucleus | protein metabolism | 132 | none |
40S ribosomal protein S4 | P62701 | 94 | 4% | 1 | cytosol-nucleus | protein metabolism | none | 53, 120 |
Tubulin beta-1 chain | Q9H4B7 | 92 | 8% | 5 | cytosol | cytoskeleton | none | 55, 106, 222, 310 |
Complement C1q subcomponent subunit A | P02745 | 86 | 6% | 2 | secreted | blood coagulation | none | 144 |
ATP-dependent RNA helicase A | Q08211 | 83 | 3% | 2 | cytosol | nucleobase metabolism | none | 9,21,43,45,149,200,218,616,727748,1155,1167,1189,1194, 1234 |
Galactokinase | P51570 | 69 | 4% | 1 | cytosol | energy metabolism | none | 47,318 |
40S ribosomal protein S2 | P60866 | 69 | 5% | 2 | cytosol-nucleus | protein metabolism | none | none |
Beta-casein | P05814 | 65 | 8% | 2 | secreted | transport | none | 32,109 |
Phosphoenolpyruvate carboxykinase | P35558 | 64 | 2% | 1 | mitochondrion | energy metabolism | none | 165,279,595 |
40S ribosomal protein S3 | P23396 | 64 | 3% | 1 | cytosol-nucleus | protein metabolism | none | 36,87,107, 67 |
Alpha-fetoprotein | P02771 | 62 | 3% | 1 | secreted | transport | none | 60, 151, 164, 426, 505, 521 |
ATP synthase beta chain | P06576 | 50 | 3% | 1 | mitochondrion | energy metabolism | none | 247, 418 |
Tubulin alpha-ubiquitous chain | P68363 | 48 | 2% | 1 | cytosol | cytoskeleton | 272 | 210,224,399,432,451 |
Heterogeneous nuclear ribonucleoprotein AB | Q53F64 | 48 | 4% | 1 | cytosol-nucleus | protein metabolism | none | 235,272,307 |
Vimentin | P08670 | 44 | 4% | 2 | cytosol | cytoskeleton | 116 | 10,29,52,275,290 |
Histone H1.3 | P16402 | 44 | 11% | 1 | nucleus | nucleobase metabolism | none | none |
Fibrinogen gamma chain | P02679 | 42 | 3% | 1 | secreted | blood coagulation | none | 27,140,288,300,444,448 |
Alpha-tropomyosin | P09493 | 39 | 1% | 1 | cytosol | cytoskeleton | none | 60,162,214,221,261,267 |
ATP synthase alpha chain | P25705 | 39 | 2% | 1 | mitochondrion | energy metabolism | none | 299,476 |
60S ribosomal protein L4 | P36578 | 37 | 3% | 1 | cytosol-nucleus | protein metabolism | none | 160 |