Figure 4
From: Proteomic analysis of plasma membrane and secretory vesicles from human neutrophils
Immunochemical analysis of specific proteins in PMV- and SV-enriched fractions. Plasma membrane vesicles (PMV) and secretory vesicles (SV) fractions were separated by SDS-PAGE, electroblotted and probed with antibodies against several proteins: (A) Lyn and Flotillin, (B) FLAP, and (C) Dysferlin. The lower molecular weight band visualized in panel C of both lanes does not correspond to the expected size for dysferlin and may be the result of limited proteolysis of dysferlin during organelle isolation or a non-specific immunoreactive protein unrelated to dysferlin.