Table 1 Differentially expressed proteins identified by proteomics from retinas of EAAC1-/- (EAAC1KO) and wild type ICR mice retina. a) P1–P14, ID number of spots. b) Probability, sequence coverage, and theoretical value of pI and Mr were obtained from the Pro Found search. The calculation of experimental pI and Mr was based on the migration of the protein on a 2D gel. c) Changes present the EAAC1-/- mice retinal protein volume ratio compared with that of ICR mice. (+/-; EAAC1-/- mice only, -/+; wild type ICR mice only)
From: Proteomic analyses of retina of excitatory amino acid carrier 1 deficient mice
a) | Â | Â | b) | Â | Â | Â | Â | Â | Â | Â | c) | Â |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Â | Â | Â | Â | Â | Â | Â | Theoretical value | Experimental value | Â | Changes | ||
| Â | Spot | Protein | Â | Probability | Sequence coverage(%) | ncbi ID | pI | Mr (kDa) | pI | Mr (kDa) | Â | EAAC1KO/ICR |
Spots identified only in the EAAC1 KO mice retina | ||||||||||||
| Â | P1 | gamma-aminobutyric acid (GABA-A) receptor, subunit beta1 | Â | 1.0e + 000 | 17 | NP_032095 | 9.1 | 54.37 | 8.9 | 56 | Â | +/- |
| Â | P2 | p300 transcriptional cofactor JMY | Â | 1.0e + 000 | 12 | NP_001004185 | 6.5 | 82.04 | 9.2 | 72 | Â | +/- |
| Â | P3 | LEK1 | Â | 1.0e + 000 | 6 | AAF07196 | 4.9 | 284.46 | 5.9 | 210 | Â | +/- |
| Â | P4 | unnamed protein product | Â | 9.9e - 001 | 16 | BAB22659 | 6.0 | 71.53 | 7.9 | 78 | Â | +/- |
| Â | Â | ANK repeat and LEM domain containing | Â | Â | Â | Q6P1H6 | Â | Â | Â | Â | Â | Â |
| Â | P5 | unnamed protein product | Â | 1.0e + 000 | 12 | BAB30425 | 10.1 | 57.84 | 8.8 | 66 | Â | +/- |
| Â | Â | Zin finger 329 | Â | Â | Â | NP_080322 | Â | Â | Â | Â | Â | Â |
| Â | P6 | RIKEN cDNA 2600017A12 | Â | 1.0e + 000 | 19 | AAH37711 | 4.3 | 86.68 | 8.8 | 74 | Â | +/- |
| Â | Â | HIV TAT Spec factor 1 | Â | Â | Â | NP_082518 | Â | Â | Â | Â | Â | Â |
| Â | P7 | striated muscle activator of Rho-dependent signaling | Â | 1.0e + 000 | 34 | AAM28877 | 6.8 | 42.98 | 5.9 | 44 | Â | +/- |
| Â | P8 | Alpha platelet-derived growth factor receptor precursor | Â | 1.0e + 000 | 13 | P26618 | 5.0 | 123.68 | 6.0 | 112 | Â | +/- |
Spots identified only in the ICR mice retina | ||||||||||||
| Â | P9 | Galnt7 protein | Â | 1.0e + 000 | 24 | AAH49907 | 6.4 | 42.70 | 4.9 | 34 | Â | -/+ |
| Â | P10 | TPA : regulator of sex-limitation 2 | Â | 1.0e + 000 | 29 | DAA01848 | 9.8 | 59.05 | 9.6 | 56 | Â | -/+ |
| Â | P11 | mKIAA0626 protein | Â | 1.0e + 000 | 21 | BAC65614 | 4.8 | 39.57 | 7.1 | 38 | Â | -/+ |
| Â | Â | microfibrillar associated protein 3 like | Â | Â | Â | NP_082032 | Â | Â | Â | Â | Â | Â |
Spots identified in both EAAC1 KO and ICR mice retina | ||||||||||||
Spots with high expression in ICR mice retina | ||||||||||||
| Â | P12 | hypothetical protein | Â | 9.9e - 001 | 23 | XP_357614 | 8.6 | 27.86 | 4.0 | 26 | Â | 0.11 |
| Â | P13 | unnamed protein product | Â | 1.0e + 000 | 40 | BAC26443 | 12.2 | 29.52 | 7.4 | 29 | Â | 0.14 |
Spots with high expression in EAAC1 KO mice retina | ||||||||||||
| Â | P14 | TPA : regulator of sex-limitation 2 | Â | 8.8e - 001 | 23 | DAA01848 | 9.8 | 59.05 | 8.3 | 56 | Â | 7.06 |