Figure 2

Localization of known raft and non-raft marker proteins in sucrose gradients. Post-nuclear homogenates (PNH) from neonatal brain tissues were extracted using 1% of Triton X-100 (TX) and fractionated in 5–40% discontinuous sucrose-density gradient as described in Methods. Twelve fractions of each 1.0 ml were collected from the top to bottom. Twenty μg of PNH protein (H) and equal 30 μl of each fraction of gradient were subjected to immunoblotting with antibodies against indicated proteins.