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Figure 1 | Proteome Science

Figure 1

From: SELDI-TOF-MS ProteinChip array profiling of T-cell clones propagated in long-term culture identifies human profilin-1 as a potential bio-marker of immunosenescence

Figure 1

Surface-enhanced laser desorption/ionisation profiles of early and late passage T-cell clones derived from octogenarian donors. CD4+ human T-cell clones from activated peripheral blood lymphocytes of healthy octogenarian donors were obtained by limiting dilution in the presence of IL-2. Protein profiling was assessed at timepoints as early as possible in antigen-stimulated in vitro culture (at the first population doubling (PD) in which two million cells were available for analysis) and approaching replicative senescence (at the penultimate or final PD before clones ceased to replicate). SELDI-TOF-MS analysis was performed using H50 reversed-phase ProteinChips. Spectra shown are the representative of early passage and late-passage T-cell clones. Normalised mass (m/z) for each peak (in Daltons (Da)) is demonstrated on the X-axis, while intensity is plotted on the Y-axis.

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Proteome Science

ISSN: 1477-5956