Figure 3

Differentially expressed protein/peptide peaks within the region of 14.5–16.5 kDa. Early-passage and late-passage T-cell clones were clustered into two comparative groups for analysis, for a total of 15 samples in each of the two groups, analyzed in triplicate. From the list of peak clusters created between 14.5 kDa and 16.5 kDa m/z, p-values were calculated using CiphergenExpress Client software (version 3.0.5.013). Peaks were manually scored for quality and peaks with signal:noise ratios (S/N) <5.0 in more than 50% of samples were removed from analysis. SELDI analysis identified two protein/peptide peaks that were differentially expressed between early and late passage T-cell clones. p < 0.05 was considered statistically significant. Molecular weight (Da), p-value, average mass:charge (m/z), median mass:charge (m/z), standard deviation, and CV are demonstrated for these two peaks (a). Representative early and late passage spectra (b) and gel-like view (c) are demonstrated for the region between 14.5 and 16.5 kDa.