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Figure 4 | Proteome Science

Figure 4

From: SELDI-TOF-MS ProteinChip array profiling of T-cell clones propagated in long-term culture identifies human profilin-1 as a potential bio-marker of immunosenescence

Figure 4

Comparative m/z determination of the 14.9 kDa differentially expressed protein/peptide peak. Lysates from several clones were pooled and enriched under the same reversed-phase conditions that were used in the H50 ProteinChip SELDI analysis. Reversed-phase high-performance liquid chromatography (HPLC) was performed in order to compare mass obtained from SELDI analysis to more accurate MALDI-TOF MS. HPLC-purified samples were run in parallel on SELDI-TOF (on a normal-phase ProteinChip) and MALDI-TOF mass spectrometers. One peak of 14.9 kDa identified as differentially expressed in T-cell senescence is demonstrated by MALDI-TOF (a) and SELDI-TOF (b) mass spectrometric analysis.

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ISSN: 1477-5956