Figure 3

Effects of LPS and AEBSF on expression of IL-1β, elastase inhibitor, vimentin, and ATP synthase. Protein levels from 2D gels from monocytes cultured for 16 h ± LPS ± AEBSF are shown for four spots on the gels. The Y-axis represents integrated normalized spot intensity, as calculated by PDQuest. The first bar is Time 0, a control of monocyte cell protein taken at the start of culture. The last two bars show the effect of AEBSF added at the end of the culture at 16 h. Letters designate particular spots on the gel. Results are means ± SE from the last gel run involving the last two donors; results from the first two donors were similar. LPS increased the expression of the particular form of IL-1β precursor found in spot A, but decreased the vimentin spot E and the ATP synthase spot M. AEBSF enhanced expression of elastase inhibitor in spot H. Results from all 30 differing spots are provided in Additional File 5. Representative gels ± LPS ± AEBSF, with spots identified by letter, are shown in Additional Files 1, 2, 3 and 4.