Table 1 Monocyte proteins altered by LPS or AEBSF. Sorted by Protein ID. Arrows indicate that the protein increased or decreased after LPS or AEBSF
Behavior | Spot | Swiss-prot | Protein ID | MW Gel | MW Theory | pI Gel | pI Theory |
|---|---|---|---|---|---|---|---|
↑AEBSF | K | P07355 | Annexin A2 | 34,000 | 38,604 | 8.9 | 7.57 |
↓AEBSF | Z | P07355 | Annexin A2 | 29,000 | 38,604 | 8.3 | 7.57 |
↑LPS | N | P08758 | Annexin A5 | 34,000 | 35,937 | 5.2 | 4.94 |
↑AEBSF | 3Z | P08758 | Annexin A5 | 101,000 | 35,937 | 6.4 | 4.94 |
↑AEBSF | J | P25705 | ATP synthase alpha chain, mitochondrial precursor | 50,000 | 59,751 | 8.9 | 9.16 |
↓LPS | M | P25705 | ATP synthase alpha chain, mitochondrial precursor | 26,000 | 59,751 | 8.6 | 9.16 |
↓LPS | V | P00488 | Coagulation Factor XIII A chain precursor | 90,000 | 83,267 | 6.2 | 5.75 |
↓AEBSF | Q | P31146 | Coronin-1A | 50,000 | 51,026 | 6.2 | 6.25 |
↑LPS | F | P02794 | Ferritin heavy chain (N-acetyl) | 25,000 | 21,226 | 5.7 | 5.31 |
↑AEBSF | X | P04406 | Glyceraldehyde-3-phosphate dehydrogenase, liver | 35,000 | 36,053 | 9.2 | 8.57 |
↑AEBSF | Y | P04406 | Glyceraldehyde-3-phosphate dehydrogenase, liver | 35,000 | 36,053 | 8.7 | 8.57 |
↓LPS | P | P68871 | Hemoglobin, subunit beta | 10,000 | 15,998 | 7.4 | 6.74 |
↑LPS | 4Z | P22626 | HN ribonucleoproteins A2/B1 | 34,000 | 37,430 | 9.3 | 8.97 |
↓LPS | T | P08514 | Integrin alpha-IIb precursor | 130,000 | 113,391 | 5.1 | 5.21 |
↑LPS | A | P01584 | Interleukin-1β precursor | 34,000 | 30,748 | 4.9 | 4.70 |
↑LPS | B | P01584 | Interleukin-1β precursor | 35,000 | 30,748 | 4.8 | 4.70 |
↑AEBSF | H | P30740 | Leukocyte Elastase Inhibitor | 40,000 | 42,742 | 6.2 | 5.90 |
↓AEBSF | W | P07737 | Profilin | 12,000 | 15,054 | 7.9 | 8.44 |
↑AEBSF | C | P14618 | Pyruvate Kinase, isozymes M1/M2 | 57,000 | 57,937 | 8.4 | 7.96 |
↑AEBSF | I | P14618 | Pyruvate Kinase, isozymes M1/M2 | 60,000 | 57,937 | 9.0 | 7.96 |
↓AEBSF | R | P14618 | Pyruvate Kinase, isozymes M1/M2 | 36,000 | 57,937 | 6.0 | 7.96 |
↓AEBSF | 5Z | P14618 | Pyruvate Kinase, isozymes M1/M2 | 60,000 | 57,937 | 7.0 | 7.96 |
↓LPS | 2Z | Q15404 | Ras suppressor protein 1 | 30,000 | 31,540 | 9.1 | 8.57 |
↑LPS | G | P04179 | Superoxide dismutase – Mn | 25,000 | 24,722 | 7.4 | 8.35 |
↓AEBSF | L | P29401 | Transketolase | 57,000 | 67,878 | 8.1 | 7.58 |
↑AEBSF | U | P29401 | Transketolase | 70,000 | 67,898 | 8.4 | 7.58 |
↓LPS | D | P09493 | Tropomyosin alpha 1 chain | 32,000 | 32,709 | 4.9 | 4.69 |
↑LPS | O | P08670 | Vimentin | 50,000 | 53,652 | 5.0 | 5.06 |
↑LPS | S | P08670 | Vimentin | 48,000 | 53,652 | 4.9 | 5.06 |
↓LPS | E | P08670 | Vimentin (amino half) | 24,000 | 53,652 | 4.6 | 5.06 |