Table 2 Differentially regulated proteins in the SM5 tumour

79

Vinculin VINC_MOUSE

SLGEIAALTSK (433–443)

2

29*

101,649

6.64

Up

298/300/302/309/311

Albumin ALBU_BOVIN

LVNELTEFAK (66–75)

2

569/623/943/164/505

64.8–65.6 (kDa)

5.92–6.37

Up

SLHTLFGDELCK (89–100)

2

LKPDPNTLCDEFKADEK (139–155)

3

YLYEIAR (161–167)

2

LSQKFPK (242–248)

2

AEFVEVTK (249–256)

2

YICDNQDTISSK (286–297)

2

SHCIAEVEK (310–318)

2

EYEATLEECCAK (375–386)

2

HLVDEPQNLIK (402–412)

2

LGEYGFQNALIVR (421–433)

2

KVPQVSTPTLVEVSR (437–451)

2/3

VPQVSTPTLVEVSR (438–451)

2

LCVLHEK (483–489)

2

CCTESLVNR (499–507)

2

QTALVELLK (549–557)

2

TVMENFVAFVDK (569–580)

2

CCAADDKEACFAVEGPK (581–597)

3

308

α-2HS-glycoprotein FETUA_BOVIN

HTLNQIDSVK (58–67)

2

519

63.2 (kDa)

3.58

Up

QQTQHAVEGDCDIHVLK (104–120)

3

QDGQFSVLFTK (121–131)

2

CDSSPDSAEDVR (132–143)

2

CDSSPDSAEDVRK (132–144)

2/3

EVVDPTKCNLLAEK (212–225)

3

CNLLAEK (219–225)

2

ALGGEDVR (238–245)

2

TPIVGQPSIPGGPVR (334–348)

2

353

Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A α isoform 2AAA_MOUSE

LAGGDWFTSR (134–143)

2

138/70

61,309

4.97

Up

MAGDPVANVR (527–536)

2

LTQDQDVDVK (566–575)

2

385

Prolyl 4-hydroxylase α-1 subunit P4HA1_MOUSE

LQDTYNLDTNTISK (137–150)

2

61/140

59,705

6.06

Up

RPCTLSELE (526–534)

2

521

Ig α-1 chain C region IGHA1_HUMAN/Lysozyme C LYSC_HUMAN/Deleted in malignant brain tumours 1 protein DMBT1_HUMAN

DASGVTFTWTPSSGK (154–168)

2

181

50,620

6.15

Up

WLQGSQELPR (264–273)

2

QEPSQGTTTFAVTSILR (283–299)/

2

STDYGIFQINSR (69–80)/

2

87

FGQGSGPIVLDDVR (73–86)

2

77

584

α-enolase ENOA_Mouse

GNPTVEVDLYTAK (15–27)

2

241

47,174

6.83

Up

AAVPSGASTGIYEALELR (32–49)

2

YITPDQLADLYK (269–280)

2

VNQIGSVTESLQACK (343–357)

2

YNQILR (406–411)

2

IEEELGSK (412–419)

2

592

α-enolase ENOA_Mouse

AAVPSGASTGIYEALELR (32–49)

2

179

46,971

7.03

Up

YITPDQLADLYK (269–280)

2

DATNVGDEGGFAPNILENK (202–220)

2

641

ERC-55 RCN2_MOUSE

VIDFDENTALDDTEEGSFR (133–151)

2

31*

44,221

3.51

Up

650

Eukaryotic translation initiation factor 3 subunit 4 IF34_MOUSE

VTNLSEDTR (243–251)

2

53

44,126

5.83

Up

814

Eukaryotic translation initiation factor 2 subunit 1 IF2A_MOUSE

VVTDTDETELAR (276–287)

2

81

37,463

5.12

Up

1134

Triosephosphate isomerase TPIS_MOUSE

VVLAYEPVWAIGTGK (159–173)^b

2

37

25,173

8.28

Up

1384

Nucleoside diphosphate kinase B NDKB_MOUSE

GDFCIQVGR (91–99)

2

46

16,433

8.66

Up

472

UV excision repair protein RAD23 homolog B RD23B_MOUSE/Endoplasmin ENPL_MOUSE

IDIDPEETVK (15–24)

2

51

53,046

4.41

Up

ASFNNPDR (213–220)/

2

SILFVPTSAPR (305–315)

2

83

GVVDSDDLPLNVSR (355–368)

2

707/730

Actin ACTB_MOUSE/

UBA/UBX 33.3 kDa protein U33K_MOUSE

AGFAGDDAPR (1–10)

2

156

41,158/40,562

5.19/5.23

Down

GYSFTTTAER (179–188)

2

SYELPDGQVITVGNER (218–233)/

LPDGTSLTQTFR (220–231)

2

74

761

Poly(rC)-binding protein 1 PCBP1_MOUSE

INISEGNCPER (47–57)

2

110

39,587

7.46

Down

IANPVEGSSGR (315–325)

2

972

Proteasome inhibitor PI31 subunit PSMF1_MOUSE

VLIDPSSGLPNR (220–231)

2

43

31,039

4.68

Down

997

Histone H4 H4_MOUSE

ISGLIYEETR (46–55)

2

32*

30,490

7.22

Down

1031

ATP synthase α chain, mitochondrial ATPA_MOUSE

VLSIGDGIAR (74–83)

2

86

29,286

9.32

Down

VVDALGNAIDGK (150–161)

2