Table 5 Functional annotation clustering of proteins involved in the transformation of tumour cell line SM7

GTP binding

Interacting selectively with GTP, guanosine triphosphate.

TBB3

0.0073

ARF1

PURA2

RHOA

Cytoskeleton

Any of the various filamentous elements that form the internal framework of cells, and typically remain after treatment of the cells with mild detergent to remove membrane constituents and soluble components of the cytoplasm. The term embraces intermediate filaments, microfilaments, microtubules, the microtrabecular lattice, and other structures characterized by a polymeric filamentous nature and long-range order within the cell. The various elements of the cytoskeleton not only serve in the maintenance of cellular shape but also have roles in other cellular functions, including cellular movement, cell division, endocytosis, and movement of organelles

COF1

0.0012

TBB3

TACC3

ARP3

RHOA

ACTB

Cellular process

Processes that are carried out at the cellular level, but are not necessarily restricted to a single cell. For example, cell communication occurs among more than one cell, but occurs at the cellular level.

PSB9

0.047

TBB3

PURA2

ROAA

PRDX2

ADA

LYPA1

FABPE

IF32

COF1

ARF1

TACC3

HPPD

RHOA

PSA3

PPID

ACTB

UBE2N

OAT

Ubiquitin-dependent protein catabolic process

The chemical reactions and pathways resulting in the breakdown of a protein or peptide by hydrolysis of its peptide bonds, initiated by the covalent attachment of a ubiquitin moiety, or multiple ubiquitin moieties, to the protein.

PSB9

0.010

PSA3

UBE2N

Intracellular transport

The directed movement of substances within a cell.

TBB3

0.042

ARF1

TACC3

RHOA

GTPase activity

Catalysis of the reaction: GTP + H₂O → GDP + phosphate.

TBB3

0.010

ARF1

ROHA

Cellular protein metabolic process

The chemical reactions and pathways involving a specific protein, rather than of proteins in general, occurring at the level of an individual cell. Includes protein modification.

PSB9

0.098

COF1

TBB3

PSA3

PPID

IF32

UBE2N

Intracellular protein transport

The directed movement of proteins in a cell, including the movement of proteins between specific compartments or structures within a cell, such as organelles of a eukaryotic cell.

ARF1

0.094

TACC3

RHOA

Negative regulation of cellular process

Any process that stops, prevents or reduces the frequency, rate or extent of cellular processes, those that are carried out at the cellular level, but are not necessarily restricted to a single cell. For example, cell communication occurs among more than one cell, but occurs at the cellular level.

TACC3

0.19

RHOA

PRDX2

Regulation of cellular process

Any process that modulates the frequency, rate or extent of cellular processes, those that are carried out at the cellular level, but are not necessarily restricted to a single cell. For example, cell communication occurs among more than one cell, but occurs at the cellular level.

COF1

0.53

TACC3

RHOA

ROAA

PRDX2

Ion binding

Interacting selectively with ions, charged atoms or groups of atoms.

PURA2

0.93

HPPD

ADA