Table 1 Use of proteomics in developmental studies.

Zebrafish

72 (hatching) and 120 hpf (larvae)

2D LC ESI-MS/MS Temporal proteome

1112 pr. (72 hpf) 867 pr. (120 hpf)

[70]

Zebrafish

72 (hatching) and 120 hpf (larvae)

2D PAGE MALDI TOF/TOF Temporal proteome

348 pr. (72 hpf) 317 pr. (120 hpf)

[70]

Zebrafish

24 hpf

SCX-TiO2-LC MS/MS Phosphoproteome

604 phoshorylated pr.

[90]

Zebrafish

24 hpf wild-type and Fyn/Yes knock down embryos

SCX-TiO₂-LC MS/MS Phosphoproteome

141 pr., differentially phosphorylated

[91]

Zebrafish

7 hpf (early gastrula)

DIGE LC MS/MS Tissue proteome

35 differentially expressed pr. between mesoderm and ectoderm

[75]

Zebrafish

6h-1 week pf (pregastrula-larvae stage)

2D PAGE MALDI TOF/TOF Temporal proteome

55 differentially expressed pr.

[69]

Zebrafish

2dpf, cloche mutant and wild-type

2D PAGE MS/MS (-)

γ-cristallin downregulated in cloche mutants

[122]

Drosophila

0 h–24 h (combined)

SCX-IMAC-LC MS/MS Phosphoproteome

2702 phosphorylated pr.

[89]

Drosophila

Embryos (0–22 h), adult heads

SCX LC MS/MS Temporal proteome

660 respectively 780 pr., 307 pr. in both stages

[74]

Drosophila

gastrula-stages

DIGE MALDI TOF/TOF Tissue proteome

37 differentially expressed pr. between lateral and ventral tissue

[76]

C.elegans

Mixed stages

SCX LC-MS/MS Purified proteoglycans

9 novel chondroitin proteoglycans

[94]

C.elegans

embryo, L1-L4 larvae, adult

DIGE MALDI TOF Temporal proteome

165 pr. expression profile

[123]

chick

Matrix vesicles

SDS-PAGE LC MS/MS Organelle proteome

126 pr.

[93]

chick

ED 7 and 11 retina tissue

2D PAGE MALDI TOF Temporal proteome

13 pr., differentially expressed

[72]

chick

Stage 29 (6 days)

2D PAGE MALDI TOF Tissue proteome

105 pr.

[124]

Rat

ED 11.5 embryo vs. Yolk sac membrane

2D PAGE MALDI MS/MS Tissue proteome

430 tissue specific pr. spots

[77]

mouse

Brain tissue ED 9.5, 11.5, 13.5

DIGE ESI MS/MS Temporal proteome

195 pr. differentially regulated

[125]

mouse

Brain tissue ED 16 and postnatal

2D PAGE MS Tissue proteome

~10 pr.

[71]

mouse

Brain tissue ED 16,5

SCX LC MS/MS Phosphoproteome

546 phosphorylation sites

[92]

mouse

ED 8.5, 9.5, 10.5 neural tube closure

2D PAGE ESI MS/MS Temporal proteome

14 pr. upregulated at ED 10.5

[73]

Sea urchin

Egg, pre-post-fertilized

2D PAGE MS/MS Phosphoproteome

94 pr. show changes after fertilization

[78]

Artemia

Diapause

2D PAGE LS MS/MS Temporal proteome

33 pr.

[126]

Artemia

Postdiapaused cysts (0–20 h)

2D PAGE MALDI TOF/TOF Temporal proteome

75 pr. differentially expressed

[127]

Rana catesbeiana

Tadpole (tailfin)

2D-PAGE or iTRAQ-MS/MS Temporal proteome

17 pr. differentially expressed after Thyroid treatment

[79]