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Table 1 Use of proteomics in developmental studies.

From: Deciphering animal development through proteomics: requirements and prospects

Model Embryos, Stage(s) Characterization Results Source
Zebrafish 72 (hatching) and 120 hpf (larvae) 2D LC ESI-MS/MS Temporal proteome 1112 pr. (72 hpf) 867 pr. (120 hpf) [70]
Zebrafish 72 (hatching) and 120 hpf (larvae) 2D PAGE MALDI TOF/TOF Temporal proteome 348 pr. (72 hpf) 317 pr. (120 hpf) [70]
Zebrafish 24 hpf SCX-TiO2-LC MS/MS Phosphoproteome 604 phoshorylated pr. [90]
Zebrafish 24 hpf wild-type and Fyn/Yes knock down embryos SCX-TiO2-LC MS/MS Phosphoproteome 141 pr., differentially phosphorylated [91]
Zebrafish 7 hpf (early gastrula) DIGE LC MS/MS Tissue proteome 35 differentially expressed pr. between mesoderm and ectoderm [75]
Zebrafish 6h-1 week pf (pregastrula-larvae stage) 2D PAGE MALDI TOF/TOF Temporal proteome 55 differentially expressed pr. [69]
Zebrafish 2dpf, cloche mutant and wild-type 2D PAGE MS/MS (-) γ-cristallin downregulated in cloche mutants [122]
Drosophila 0 h–24 h (combined) SCX-IMAC-LC MS/MS Phosphoproteome 2702 phosphorylated pr. [89]
Drosophila Embryos (0–22 h), adult heads SCX LC MS/MS Temporal proteome 660 respectively 780 pr., 307 pr. in both stages [74]
Drosophila gastrula-stages DIGE MALDI TOF/TOF Tissue proteome 37 differentially expressed pr. between lateral and ventral tissue [76]
C.elegans Mixed stages SCX LC-MS/MS Purified proteoglycans 9 novel chondroitin proteoglycans [94]
C.elegans embryo, L1-L4 larvae, adult DIGE MALDI TOF Temporal proteome 165 pr. expression profile [123]
chick Matrix vesicles SDS-PAGE LC MS/MS Organelle proteome 126 pr. [93]
chick ED 7 and 11 retina tissue 2D PAGE MALDI TOF Temporal proteome 13 pr., differentially expressed [72]
chick Stage 29 (6 days) 2D PAGE MALDI TOF Tissue proteome 105 pr. [124]
Rat ED 11.5 embryo vs. Yolk sac membrane 2D PAGE MALDI MS/MS Tissue proteome 430 tissue specific pr. spots [77]
mouse Brain tissue ED 9.5, 11.5, 13.5 DIGE ESI MS/MS Temporal proteome 195 pr. differentially regulated [125]
mouse Brain tissue ED 16 and postnatal 2D PAGE MS Tissue proteome ~10 pr. [71]
mouse Brain tissue ED 16,5 SCX LC MS/MS Phosphoproteome 546 phosphorylation sites [92]
mouse ED 8.5, 9.5, 10.5 neural tube closure 2D PAGE ESI MS/MS Temporal proteome 14 pr. upregulated at ED 10.5 [73]
Sea urchin Egg, pre-post-fertilized 2D PAGE MS/MS Phosphoproteome 94 pr. show changes after fertilization [78]
Artemia Diapause 2D PAGE LS MS/MS Temporal proteome 33 pr. [126]
Artemia Postdiapaused cysts (0–20 h) 2D PAGE MALDI TOF/TOF Temporal proteome 75 pr. differentially expressed [127]
Rana catesbeiana Tadpole (tailfin) 2D-PAGE or iTRAQ-MS/MS Temporal proteome 17 pr. differentially expressed after Thyroid treatment [79]
  1. A summary of published results on the use of proteomics in different model systems of developmental biology (pf = postfertilization, pr = proteins)