Figure 6

SELDI analysis of hepcidin mixes. a) spectra of serum with 200 ng/ml labelled hepcidin (L) added in addition to 67–400 ng/ml unlabelled hepcidin (U) as indicated in the grey boxes. b) spectra of labelled (dotted line) and unlabelled (solid line) hepcidin at 200 ng/ml in serum and the sum of the 2 spectra (bold line). c) shows the concentration of unlabelled hepcidin calculated from the peak height ratio plotted against the actual concentration in the experiment of Figure 6a and two independent replicates in different sera. The trend line represents a least-squares linear regression (r² = 0.990).