Table 1 List of proteins identified from the 2D gel shown in figure 1 following in-gel tryptic digestion, MALDI-MS analysis, and database searching.
From: Histoplasma capsulatum proteome response to decreased iron availability
Spot # | BLAST ID | # Peptides Matched/#Searched | % Protein Coverage | Accession # | MASCOT Score (p < .05) |
|---|---|---|---|---|---|
Spot 1* | predicted protein [Ajellomyces capsulatus NAm1]. | 8/17 | 12% | XP_001538505 | 71 |
Spot 2 | aconitate hydratase, mitochondrial precursor [Ajellomyces capsulatus NAm1]. | 13/29 | 14% | XP_001539799 | 107 |
Spot 3 | heat shock 70 kDa protein C precursor [Ajellomyces capsulatus NAm1]. | 12/17 | 21% | XP_001538200 | 150 |
Spot 4 | heat shock protein 70 [Paracoccidioides brasiliensis]. | 12/16 | 17% | AAK66771 | 162 |
Spot 5 (A10) | 70 kDa heat shock protein [Paracoccidioides brasiliensis]. | 12/26 | 20% | AAP05987 | 125 |
Spot 6* | protein disulfide-isomerase precursor [Ajellomyces capsulatus NAm1]. | 6/9 | 12% | XP_001541532 | 77 |
Spot 7 | heat shock 70 kDa protein [Ajellomyces capsulatus NAm1]. | 7/10 | 14% | XP_001543760 | 90 |
Spot 8 | heat shock protein 60, mitochondrial precursor [Ajellomyces capsulatus NAm1]. | 18/26 | 34% | XP_001539356 | 242 |
Spot 9 | ATP synthase beta chain, mitochondrial precursor [Ajellomyces capsulatus NAm1]. | 13/24 | 37% | XP_001539339 | 159 |
Spot 10 (A21) | enolase [Paracoccidioides brasiliensis]. | 13/21 | 37% | ABQ45367 | 172 |
Spot 11 | ubiquinol-cytochrome C reductase complex core protein 2, putative [Aspergillus fumigatus Af293]. | 9/22 | 25% | XP_001539391 | 103 |
Spot 12 | dihydrolipoamide dehydrogenase [Ajellomyces capsulatus NAm1]. | 8/11 | 17% | XP_747922 | 119 |
Spot 13* | fumarate hydratase class II [Aspergillus terreus NIH2624]. | 8/20 | 16% | XP_001209891 | 91 |
Spot 14*(A29) | saccharopine dehydrogenase [Ajellomyces capsulatus NAm1]. | 6/16 | 14% | XP_001544528 | 64 |
Spot 15 | elongation factor Tu, mitochondrial precursor [Aspergillus terreus NIH2624]. | 12/21 | 38% | XP_001210502 | 170 |
Spot 16 | pyruvate dehydrogenase E1 component alpha subunit, mitochondrial precursor [Ajellomyces capsulatus NAm1]. | 9/18 | 21% | XP_001544313 | 106 |
Spot 17 (A38) | malate dehydrogenase [Ajellomyces capsulatus NAm1]. | 7/17 | 21% | XP_001541871 | 86 |
Spot 18 | glyceraldehyde-3-phosphate dehydrogenase [Ajellomyces capsulatus]. | 8/16 | 35% | AAG33368 | 112 |
Spot 19 | ketol-acid reductoisomerase, mitochondrial precursor [Ajellomyces capsulatus NAm1]. | 20/37 | 46% | XP_001536226 | 258 |
Spot 20 | mannitol-1-phosphate dehydrogenase [Paracoccidioides brasiliensis]. | 16/22 | 44% | AAO47089 | 234 |
Spot 21 | conserved hypothetical protein [Ajellomyces capsulatus NAm1]. | 9/18 | 25% | XP_001544536 | 118 |
Spot 22 | peptidyl-prolyl cis-trans isomerase [Ajellomyces capsulatus NAm1]. | 14/30 | 35% | XP_001536169 | 170 |
Spot 23 | peptidyl-prolyl cis-trans isomerase [Aspergillus fumigatus Af293]. | 14/31 | 27% | XP_001536169 | 100 |
Spot 24* | fructose 1,6-biphosphate aldolase 1 [Paracoccidioides brasiliensis]. | 4/7 | 8% | AAL34519 | 62 |
Spot 25* | RACK1-like protein [Paracoccidioides brasiliensis]. | 6/16 | 17% | ABA33785 | 76 |
Spot 26* | cytochrome c peroxidase Ccp1 [Aspergillus fumigatus Af293]. | 9/28 | 25% | XP_751914 | 102 |
Spot 27* | protein disulfide-isomerase precursor [Ajellomyces capsulatus NAm1]. | 5/12 | 7% | XP_001541532 | 68 |
Spot 28 | gene encoding Histoplasma capsulatum predicted protein (2481 nt) | 5/12 | 5% | HCAG_01143 | 65 |
Spot 29* | mRNA binding post-transcriptional regulator (Csx1) [Aspergillus fumigatus Af293]. | 5/13 | 16% | XP_746709 | 66 |
Spot 30* | gene encoding Histoplasma capsulatum predicted protein (2328 nt) | 5/16 | 10% | HCAG_06550 | 68 |
Spot 31* | hypothetical protein RUMOBE_03015 [Ruminococcus obeum ATCC 29174] | 5/9 | 9% | ZP_01965283 | 74 |
Spot 32 | HCAG_04492 | 4/7 | 7% | - | 60 |
Spot 33 | Hsp90 binding co-chaperone (Sba1) [Aspergillus fumigatus Af293]. | 6/18 | 19% | XP_753264 | 72 |
Spot 34 | thiol-specific antioxidant [Ajellomyces capsulatus NAm1]. | 10/21 | 38% | XP_001538605 | 142 |
Spot 35* | triosephosphate isomerase [Aspergillus clavatus NRRL 1]. | 7/19 | 33% | XP_001274623 | 103 |
Spot 36 | 30 kDa heat shock protein [Ajellomyces capsulatus NAm1]. | 10/28 | 31% | XP_001540271 | 125 |
Spot 37 | superoxide dismutase, mitochondrial precursor [Ajellomyces capsulatus NAm1]. | 5/16 | 21% | XP_001541351 | 78 |
Spot 38 | ATP synthase D chain, mitochondrial [Ajellomyces capsulatus NAm1]. | 7/19 | 31% | XP_001539631 | 102 |
Spot 39 | tropomyosin, putative [Neosartorya fischeri NRRL 181]. | 6/12 | 27% | XP_001261343 | 98 |
Spot 40* | protein of unknown function DUF181 [Methylobacterium radiotolerans JCM 2831]. | 5/9 | 14% | YP_001756253 | 76 |
Spot 41 | peptidyl-prolyl cis-trans isomerase (cyclophilin) [Ajellomyces capsulatus NAm1]. | 7/24 | 26% | XP_001540645 | 91 |
Spot 42 | putative cytochrome c oxidase subunit VIa [Paracoccidioides brasiliensis]. | 5/14 | 13% | AAT77146 | 76 |
Spot 43 | woronin body major protein [Ajellomyces capsulatus NAm1]. | 4/8 | 15% | XP_001543637 | 63 |
Spot 44 (A5) | HCAG_04147 (Hypothetical Protein) | 5/10 | 9% | - | 66 |
Spot 45*(A42) | asparaginyl-tRNA synthetase [Flavobacterium sp. MED217]. | 6/18 | 16% | ZP_01059347 | 65 |
Spot 46 | ATPase associated with various cellular activities AAA_3 [Shewanella woodyi ATCC 51908] | 6/16 | 11% | YP_001761394 | 67 |
Spot 47* | cystathionine beta-lyase [Rhizobium etli CFN 42]. | 6/14 | 13% | YP_469412 | 62 |
Spot 48 | heat shock protein 70 [Paracoccidioides brasiliensis]. | 7/11 | 12% | AAK66771 | 120 |
Spot 49 | hypothetical protein NCU07591 [Neurospora crassa OR74A]. | 4/7 | 7% | XP_962971 | 64 |