Table 3 Yield of soluble recombinant protein. Results sorted according to ORF size [kDa].
From: Automated production of recombinant human proteins as resource for proteome research
ORF size [kDa] | ORF count [n] | purif. ORF [n] | Yield ORF [%] | Hisa Hisb | NusAa Hisb | GSTa GSTb | MBPa MBPb | MBPa Hisb |
|---|---|---|---|---|---|---|---|---|
0–9 | 2 | 1 | 50 | 1 | 0 | 0 | 0 | 1 |
10–19 | 10 | 9 | 90 | 4 | 8 | 8 | 5 | 7 |
20–29 | 21 | 15 | 70 | 6 | 11 | 8 | 6 | 14 |
30–39 | 15 | 10 | 70 | 2 | 8 | 4 | 3 | 6 |
40–49 | 15 | 10 | 70 | 3 | 10 | 6 | 4 | 7 |
50–59 | 3 | 1 | 30 | 0 | 1 | 0 | 0 | 0 |
60–69 | 8 | 3 | 40 | 2 | 1 | 0 | 0 | 0 |
70–79 | 5 | 2 | 40 | 1 | 1 | 0 | 0 | 0 |
80–89 | 7 | 2 | 30 | 0 | 2 | 0 | 0 | 1 |
90–99 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
≥ 100 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |