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Table 3 Yield of soluble recombinant protein. Results sorted according to ORF size [kDa].

From: Automated production of recombinant human proteins as resource for proteome research

ORF size [kDa] ORF count [n] purif. ORF [n] Yield ORF [%] Hisa Hisb NusAa Hisb GSTa GSTb MBPa MBPb MBPa Hisb
0–9 2 1 50 1 0 0 0 1
10–19 10 9 90 4 8 8 5 7
20–29 21 15 70 6 11 8 6 14
30–39 15 10 70 2 8 4 3 6
40–49 15 10 70 3 10 6 4 7
50–59 3 1 30 0 1 0 0 0
60–69 8 3 40 2 1 0 0 0
70–79 5 2 40 1 1 0 0 0
80–89 7 2 30 0 2 0 0 1
90–99 6 0 0 0 0 0 0 0
≥ 100 4 0 0 0 0 0 0 0
  1. a fusion tag
  2. b tag employed for purification strategy