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Figure 1 | Proteome Science

Figure 1

From: Peptides OFFGEL electrophoresis: a suitable pre-analytical step for complex eukaryotic samples fractionation compatible with quantitative iTRAQ labeling

Figure 1

Comparison of experimental and theoretical pI for each OFFGEL fraction. (A) Average experimental pI values for all peptides identified per fraction. The dark area relates to the plasma peptides and the white area to the secretome peptides. Standard deviation is represented by errors bars. The broken lines are based on the theoretical pI values calculated according to the supplier's specifications of the IPG gel strip of 24 cm length (pH 3–10). (B) Percentage error between the average experimental pI and the average theoretical pI per fraction. Black squares correspond to the error for plasma and triangles correspond to the error for secretome.

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