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Table 2 iTRAQ labeling influence on peptides repartition for OFFGEL fractions 2, 11 and 24 for secretome sample.

From: Peptides OFFGEL electrophoresis: a suitable pre-analytical step for complex eukaryotic samples fractionation compatible with quantitative iTRAQ labeling

 

+ iTRAQ

- iTRAQ

  

Fraction

Peptide pI Average

s.d.

Peptide pI Average

s.d.

ΔpI

% of Coverage

2

4.21

0.66

4.11

0.31

0.10

68.12

11

5.88

0.53

5.88

0.36

0.00

74.14

24

8.74

1.35

9.22

1.13

-0.48

56.90

  1. (s.d. = standard deviation; ΔpI = difference of the peptide pI average for a same fraction with and without iTRAQ labeling)