Table 1 Stoichiometric ratios of protein complexes as quantitated by APEX
(a)Protein complex | (b) E. coli stoichiometric ratio | SD1 APEX ratio | (c)SD1 APEX abundances (± sd) (molecules/cell) |
|---|---|---|---|
SucC/SucD | 1:1 | 1:1.01 | 6889(± 827):7004(± 651) |
AccD/AccA | 1:1 | 1:1.03 | 2012(± 302):2086(± 121) |
AccC/AccB | 1:2 | 1:2.12 | 1534(± 549):3258(± 460) |
AceF/AceE | 1:1 | 1:1.04 | 6716(± 739):7029(± 707) |
SdhB/SdhA | 1:1 | 1:1.21 | 4869(± 740):5917(± 44) |
HslU/HslV | 1:2 | 1:1.98 | 1486(± 125):2953(± 955) |
RpoB/RpoA | 1:2 | 1:2.05 | 2095(± 301):4312(± 1016) |
AtpD/AtpA | 1:1 | 1:1.01 | 8713(± 216):8848(± 673) |
AtpH/AtpG | 1:1 | 1:1.41 | 1052(± 295):1491(± 595) |
NlpB/YaeT | 1:1 | 1:1.46 | 477(± 25):699(± 76) |
AhpF/AhpC | 1:1 or 1:5 | 1:6.02 | 2272(± 216):13673(± 303) |
YaeT/NlpB/SmpA/YfiO/YfgL | 1:1:1:1:1 | 2.8:1.9:4.6:1:10.6 | 699:477:1157:250:2655 |
AtpA/AtpD/AtpG/AtpH | 3:3:1:1 | 8.4:8.3:1.4:1 | 8848:8713:1491:1052 |