Figure 6From: A fractionation method to identify qauntitative changes in protein expression mediated by IGF-1 on the proteome of murine C2C12 myoblasts Triplicate samples from three different C2C12 cell preparations were stimulated with IGF-1 (100 ng/ml) for up to 8 hours, fractionated using batch ion-exchange, separated by 2D electrophoresis, and proteins were detected with silver stain. The protein highlighted by a yellow square (eIF-4H) rapidly increases in expression after 1 hour IGF-1 treatment, but then decreases at 4 and 8 hours.Back to article page