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Table 2 Proteins whose expression is consistently decreased after 8 hour treatment with IGF-1.

From: A fractionation method to identify qauntitative changes in protein expression mediated by IGF-1 on the proteome of murine C2C12 myoblasts

Spot Number

Protein Name

NCBI gi #

Fold Decrease

SC (%)

Z-Score

MW; pI (theoretical)

MW; pI (actual)

 

Cell surface receptor:

      

24

Platelet-derived growth factor receptor, alpha precursor (PDGF-R-alpha)

2506800

3.8 ± 0.3

11

1.30

123.68/5.0

125/4.8

 

Cytoskeletal protein:

      

24A

cofilin

6680924

4.9 ± 0.1

37

2.01

18.55/8.5

25/5.6

 

DNA topoisomerase activity:

      

25

Topoisomerase (DNA) II beta

32451610

3.6 ± 0.2

11

1.82

182.80/8.7

140/8.0

 

GTPase activating protein:

      

26

Rac GTPase activating protein; GAB-associated CDC42

28569544

3.4 ± 0.3

8

1.62

191.74/6.6

180/7.0

27

Rho GDP-dissociation inhibitor 1

31982030

3.6 ± 0.2

34

2.30

23.41/5.1

30/5.4

 

Heat shock protein (Chaperone):

      

28

Heat shock protein 1 (chaperonin)

16741093

5.8 ± 0.2

18

1.46

59.02/5.5

60/5.7

 

Protein Phosphatase:

      

29

secreted embryonic phosphatase

16580151

4.1 ± 0.4

25

1.27

55.23/6.3

60/7.8

 

Structural constituent of ribosome:

      

30

Stmn1 protein

32449851

3.2 ± 0.3

32

1.60

17.26/5.7

18/7.6

 

Transcription factor:

      

31

myelin transcription factor 1-like

6679000

3.9 ± 0.1

20

2.05

137.62/4.8

140/5.2

32

zinc finger protein 100

50510803

4.7 ± 0.4

8

1.99

104.8/9.3

100/8.5

 

Transport/Cargo:

      

33

Sorting nexin 13

38614111

3.8 ± 0.2

6

1.13

111.54/6.2

120/7.0

 

Ubiquitin proteasome system protein:

      

34

ubiquitin fusion degradation 1 like

31981443

4.3 ± 0.1

22

1.83

34.75/6.0

29/6.4

35

Ubiquitin-conjugating enzyme E2 N

46577661

3.2 ± 0.1

24

2.22

17.14/6.1

18/6.5

 

Unclassified/Unknown function:

      

36

Mapkbp1

47124622

4.0 ± 0.1

9

1.11

164.70/6.3

60/7.5

37

Phosphatidylethanolamine-BP 1

1517864

3.2 ± 0.3

17

2.31

20.83/5.2

22/5.2

38

Stromal antigen 2

41940887

3.8 ± 0.1

21

1.66

135.13/5.2

190/6.2

39

Tpr

14329713

3.7 ± 0.2

18

1.40

139.30/5.3

110/5.3

  1. Note 1: Protein classification is according to the Human Protein Reference Database http://www.hprd.org.
  2. Note 2: SC%: Percentage of sequence coverage.
  3. Note 3: Spot Number refers to the location of the individual proteins on 2D gels as shown in Figures 3–6. When a protein was identified in more than one Batch IEX fraction, it is marked multiple gels.
  4. Note 4: Supplemental Table 2 contains all MALDI TOF data generated for each protein.
  5. Note 5: Only proteins with probability score of 1.0e+000 were included in the table.
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