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Table 2 Proteins whose expression is consistently decreased after 8 hour treatment with IGF-1.

From: A fractionation method to identify qauntitative changes in protein expression mediated by IGF-1 on the proteome of murine C2C12 myoblasts

Spot Number Protein Name NCBI gi # Fold Decrease SC (%) Z-Score MW; pI (theoretical) MW; pI (actual)
  Cell surface receptor:       
24 Platelet-derived growth factor receptor, alpha precursor (PDGF-R-alpha) 2506800 3.8 ± 0.3 11 1.30 123.68/5.0 125/4.8
  Cytoskeletal protein:       
24A cofilin 6680924 4.9 ± 0.1 37 2.01 18.55/8.5 25/5.6
  DNA topoisomerase activity:       
25 Topoisomerase (DNA) II beta 32451610 3.6 ± 0.2 11 1.82 182.80/8.7 140/8.0
  GTPase activating protein:       
26 Rac GTPase activating protein; GAB-associated CDC42 28569544 3.4 ± 0.3 8 1.62 191.74/6.6 180/7.0
27 Rho GDP-dissociation inhibitor 1 31982030 3.6 ± 0.2 34 2.30 23.41/5.1 30/5.4
  Heat shock protein (Chaperone):       
28 Heat shock protein 1 (chaperonin) 16741093 5.8 ± 0.2 18 1.46 59.02/5.5 60/5.7
  Protein Phosphatase:       
29 secreted embryonic phosphatase 16580151 4.1 ± 0.4 25 1.27 55.23/6.3 60/7.8
  Structural constituent of ribosome:       
30 Stmn1 protein 32449851 3.2 ± 0.3 32 1.60 17.26/5.7 18/7.6
  Transcription factor:       
31 myelin transcription factor 1-like 6679000 3.9 ± 0.1 20 2.05 137.62/4.8 140/5.2
32 zinc finger protein 100 50510803 4.7 ± 0.4 8 1.99 104.8/9.3 100/8.5
  Transport/Cargo:       
33 Sorting nexin 13 38614111 3.8 ± 0.2 6 1.13 111.54/6.2 120/7.0
  Ubiquitin proteasome system protein:       
34 ubiquitin fusion degradation 1 like 31981443 4.3 ± 0.1 22 1.83 34.75/6.0 29/6.4
35 Ubiquitin-conjugating enzyme E2 N 46577661 3.2 ± 0.1 24 2.22 17.14/6.1 18/6.5
  Unclassified/Unknown function:       
36 Mapkbp1 47124622 4.0 ± 0.1 9 1.11 164.70/6.3 60/7.5
37 Phosphatidylethanolamine-BP 1 1517864 3.2 ± 0.3 17 2.31 20.83/5.2 22/5.2
38 Stromal antigen 2 41940887 3.8 ± 0.1 21 1.66 135.13/5.2 190/6.2
39 Tpr 14329713 3.7 ± 0.2 18 1.40 139.30/5.3 110/5.3
  1. Note 1: Protein classification is according to the Human Protein Reference Database http://www.hprd.org.
  2. Note 2: SC%: Percentage of sequence coverage.
  3. Note 3: Spot Number refers to the location of the individual proteins on 2D gels as shown in Figures 3–6. When a protein was identified in more than one Batch IEX fraction, it is marked multiple gels.
  4. Note 4: Supplemental Table 2 contains all MALDI TOF data generated for each protein.
  5. Note 5: Only proteins with probability score of 1.0e+000 were included in the table.