Figure 2

Identification of protein spots on 2D gel. Image depicts Cy3 (internal standard) channel for a representative gel. Proteins from each sample group and the internal standard were separated in two dimensions. Horizontal dimension is isoelectric focusing (pH3-10, acidic end to left). Vertical dimension is 12.5% SDS-PAGE. Indicated spots were identified with high confidence and met criteria for statistical and biological significance. Some proteins were identified more than once because charge isoforms were present.