Figure 4

Proteomic patterns. Three-dimensional scatter plot of entire proteomic data set. A. Results of cluster analysis represented as heat map. Proteomic features that changed significantly in immortalized versus HFK populations were analyzed by unsupervised cluster analysis. Experimental samples are clustered on the horizontal axis and protein spots on the vertical axis. Legend indicates fold change on log₁₀ scale. B. Three-dimensional scatter plot of entire proteomic data set. Axes represent fold change in expression due to E6 alone (2^xi), E7 alone (2^yi), and the E6/7 interaction (2^zi). Grey spots did not reach criteria for significance. Other spots denote proteins that were significantly up-regulated (yellow), down-regulated (blue), or showed a mixed pattern of regulation (turquoise) in E6- and E7-transduced populations (i.e., x _i > 0 and y _i < 0 or x _i < 0 and y _i > 0). Different forms of HSPB1 were assigned a common color (green) to aid in visualization. Charge isoforms of HSPA1 and HSPA1 are labeled according to spot number in the master spot map. Shape of symbols denotes significance of z _i (squares, not significant; circles, significant). For clarity, labels have been omitted for identified proteins in the large clusters of spots that were similarly regulated.