Figure 6
From: Identification of pancreatic cancer invasion-related proteins by proteomic analysis
A. Immunoblot of VIM knockdown in Clone #3 untreated control, scrambled control, VIM-siRNA (1), VIM-siRNA (2) and VIM-siRNA (3) ( upper ) and α-tubulin as loading control ( lower ). B. Invasion assay of Clone #3 cells after siRNA silencing of VIM. The total number of invading cells was determined by counting the number of cells per field in 10 random fields, at 200× magnification. The average number of cells per field was then multiplied by a factor of 140 (growth area of membrane/field area viewed at 200× magnification (calibrated using a microscope graticule)). Experiments performed in triplicate. Statistics; * ≤ 0.05, ** ≤ 0.01, *** ≤ 0.005.